中国组织工程研究Issue(33)：5312-5317,6.DOI:10.3969/j.issn.2095-4344.2015.33.013
附子水煎剂对家兔离体主动脉血管张力的影响
Tension effect of water decoction ofAconitum carmichaeli Debx on rabbit aortain vitro
摘要
Abstract
BACKGROUND:Aconitum carmichaeli Debx (ACD) is the tuberous root of Aconium carmicgaekum, used as cardiotonic to restore yang for the treatment of colapse and shock, to warm the kidney and reinforceyang, and to expel cold and promote the flow ofyang-qi. Studies have found that ACD has obviously cardiotonic, antihypertensive, vasodilatory, analgesic, anti-inflammatory and toxic effects. OBJESTIVE: To observe the vasodilatory effects of a water decoction of ACD on rabbit’s aorta rings and its mechanism. METHODS:Rabbits aorta arteries were isolated, pre-contracted with noradrenaline (10-6 mol/L) and their responses to different concentrations of ACD (0.5, 1.0, 2.0, 4.0, 8.0 g/L) were investigated. The effects of removal of vascular endothelium and different signaling pathway inhibitors (Nω-nitro-L-arginine: 1×10-4 mol/L, methylene blue: 1×10-5 mol/L, indomethacin: 1×10-5 mol/L, propranolol: 1×10-5 mol/L) on ACD-induced vasodilation were also assessed. RESULTS AND CONCLUSION:ACD could not change the resting tension of rabbit aortic rings, but ACD treatment resulted in an obvious relaxation in narodrenaline-precontracted aortic rings and the relaxant effect was dose-dependent. The vasodilatory effect of ACD was significantly reduced by removal of endothelium, 1×10-4 mol/L Nω-nitro-L-arginine and 1×10-5 mol/L methylene blue but not reduced by indomethacin and propranolol. In addition, 4 g/L water decoction of ACD did not decrease the dose-response curves of artery rings to narodrenaline or KCl in the absence of endothelial cels. ACD can relax isolated rabbit’s aorta, which may be related to endothelium-released nitric oxide, but has no significant relevance with receptor-operated and voltage-dependent calcium channels.关键词
组织构建/组织工程/附子/血管/内皮细胞/血管舒张/一氧化氮/血管张力/离体Key words
Aconitum/Aorta/Endothelial Cells/Vasodilatio分类
医药卫生引用本文复制引用
李红专,刘行海,牛彩琴,张团笑..附子水煎剂对家兔离体主动脉血管张力的影响[J].中国组织工程研究,2015,(33):5312-5317,6.基金项目
四川省教育厅重点项目(14ZA0182)Funding:the Key Program of Sichuan Department of Education, No.14ZA0182 （14ZA0182）
