中国病理生理杂志Issue(9)：1589-1594,6.DOI:10.3969/j.issn.1000-4718.2015.09.010
短链酰基辅酶 A 脱氢酶在心肌细胞凋亡中的作用
Effects of short-chain acyl-CoA dehydrogenase on cardiomyocyte apopto-sis
摘要
Abstract
AIM:To investigate the change of short-chain acyl-CoA dehydrogenase (SCAD) expression during cardiomyocyte apoptosis and to explore the relationship between SCAD and cardiomyocyte apoptosis .METHODS: The neonatal rat cardiomyocytes treated by tert-butyl hydroperoxide (tBHP) were used as the model of cardiomyocyte apoptosis . The cell viability , the expression of SCAD at mRNA and protein levels , the activity of SCAD and the content of free fatty acids were determined .RESULTS:The mRNA and protein expression of SCAD decreased in the cardiomyocyte apoptosis model.Compared with negative control group , SCAD expression and activity were both significantly decreased in siRNA-1186 group, but the content of free fatty acids were obviously increased in the cardiomyocytes .Meanwhile, SCAD siRNA treatment triggered the same apoptosis as cardiomyocytes treated with tBHP .CONCLUSION: Down-regulation of SCAD may play an important role in primary cardiomyocyte apoptosis .Increase in the expression of SCAD may become an impor-tant part in intervening cardiomyocyte apoptosis .关键词
短链酰基辅酶A脱氢酶/心肌细胞/细胞凋亡/心力衰竭/能量代谢/叔丁基过氧化氢Key words
Short-chain acyl-CoA dehydrogenase/Cardiomyocytes/Apoptosis/Heart failure/Energy metabo-lism/Tert-butyl hydroperoxide分类
医药卫生引用本文复制引用
曾振华,黄秋菊,黄金贤,舒朝辉,刘培庆,陈少锐,刘冰,周四桂..短链酰基辅酶 A 脱氢酶在心肌细胞凋亡中的作用[J].中国病理生理杂志,2015,(9):1589-1594,6.基金项目
国家自然科学基金青年科学基金资助项目（No．81000072）；广东省“十二五”医学重点学科，依托广东药学院附属第一医院、药科学院；广东省科技计划 （）
