中国病理生理杂志Issue(9)：1715-1719,5.DOI:10.3969/j.issn.1000-4718.2015.09.033
人参皂苷 Rh2对人骨肉瘤细胞 MG－63凋亡的影响
Effect of Ginsenoside Rh2 on apoptosis of human osteosarcoma cell line MG-63
王立民1
作者信息
- 1. 胜利石油管理局胜利医院骨科，山东东营257055
- 折叠
摘要
Abstract
AIM:To investigate the effect of Ginsenoside Rh2 (Rh2) on the apoptosis of human osteosarcoma cell line MG-63.METHODS:The cell viability was determined by MTT assay .MG-63 cell apoptotic rate was examined by flow cytometry with Annexin V-PI double staining.The expression of Bcl-2, Bax, cytochrome C ( Cyt C) and cleaved caspase-3 were measured by Western blot .RESULTS: Rh2 enhanced the apoptosis of MG-63 cells in a dose-dependent manner.Furthermore, after treatment with Rh2, the release of mitochondrial Cyt C and Bax expression were increased , while Bcl-2 and the ratio of Bcl-2/Bax were decreased as compared with control group ( P<0.05 ) .The protein level of cleaved caspase-3 was also increased (P<0.05).CONCLUSION:Ginsenoside Rh2 accelerates the apoptosis of MG-63 cells through mitochondria-dependent pathway , suggesting that Rh2 is a novel approach for the treatment of osteosarcoma .关键词
人参皂苷Rh2/人骨肉瘤细胞MG-63/细胞凋亡Key words
Ginsenoside Rh2/Human osteosarcoma cell line MG-63/Apoptosis分类
医药卫生引用本文复制引用
王立民..人参皂苷 Rh2对人骨肉瘤细胞 MG－63凋亡的影响[J].中国病理生理杂志,2015,(9):1715-1719,5.
