中国当代儿科杂志Issue(9)：989-993,5.DOI:10.7499/j.issn.1008-8830.2015.09.021
红花注射液对白血病HEL细胞增殖和凋亡的影响及相关机制探讨
Effects of saflfower injection on HEL leukemia cell proliferation and apoptosis and relevant molecular mechanisms
摘要
Abstract
Objective To study the effect of safflower injection on the proliferation and apoptosis of human leukemia cell line HEL and the relevant molecular mechanisms. Methods HEL cells were treated with different concentrations of saflfower injection. HEL cells without saflfower injection treatment were used as the control group. MTT method was used to detect the inhibitory rate of the HEL cells at 24, 48 and 72 hours after various concentrations of saflfower injection treatment (10, 20, 30, 40 and 50 mg/mL). The cell cycle and apoptosis were detected using lfow cytometry and the HOXB3-mRNA expression was measured by RT-PCR at 48 hours after saflfower injection treatment (10, 20 and 30 mg/mL). Results Compared with the control group, various concentrations of safflower injection inhibited HEL cell proliferation in a dose-dependent manner (P<0.05). At 48 hours after various concentrations of saflfower injection treatment, the number of treated cells in the G2/M phase increased, but that in the S phase decreased, and the apoptosis rate was signiifcantly higher than that in the control group, with a dose-dependent manner (P<0.05). The expression of HOXB3-mRNA in saflfower injection-treated cells decreased in a dose-dependent manner compared with the control group (P<0.05). Conclusions Saflfower injection can inhibit proliferation and induce apoptosis of HEL cells in vitro, and its underlying mechanisms may involve down-regulation of the HOXB3-mRNA expression.关键词
红花注射液/白血病/细胞增殖/细胞凋亡/HOXB3/人类白血病细胞株Key words
Saflfower injection/Leukemia/Cell proliferation/Cell apoptosis/HOXB3/Human leukemia cell line引用本文复制引用
段培锋,贾秀红,王建勇..红花注射液对白血病HEL细胞增殖和凋亡的影响及相关机制探讨[J].中国当代儿科杂志,2015,(9):989-993,5.基金项目
山东省自然科学基金项目（ZR2014HL032）；山东省医药卫生科技发展计划项目（2014WS0183）；滨州市科技发展计划项目（2014ZC0113）；滨州医学院科技计划项目（BY2013KJ03）。 （）
