中国当代儿科杂志Issue(9)：994-1000,7.DOI:10.7499/j.issn.1008-8830.2015.09.022
哮喘小鼠气道重塑过程中CD4+CD25+调节性T细胞和Th17细胞表达的动态变化
Dynamic changes in percentages of CD4+CD25+regulatory T cells and Th17 cells in process of airway remodeling in mouse model of asthma
摘要
Abstract
Objective To study the dynamic changes in Th17 cells and CD4+CD25+regulatory T cells (Treg) in the spleen and to analyze their relationship with airway remodeling. Methods A total of 48 female speciifc pathogen-free Balb/c mice were randomly divided into control and asthmatic groups. To establish the asthmatic airway remodeling model, the mice were sensitized to ovalbumin (OVA) through intraperitoneal injection of OVA and aluminum hydroxide suspension and challenged by inhalation of aerosol OVA. The matched control group was treated with normal saline instead. In 24 hours after 2-week, 4-week, and 8-week aerosol inhalation, 8 mice were randomly selected from each group and sacriifced. Then histopathological examination of the left lung was performed to measure the degree of airway remodeling. The percentages of Th17 and CD4+CD25+Treg cells in total CD4+cells from the spleen were determined by lfow cytometry. Results In the asthmatic group, the ratios of total bronchial wall area to bronchial basement membrane perimeter (WAt/Pbm) and bronchial smooth muscle area to bronchial basement membrane perimeter (WAm/Pbm) signiifcantly increased as the challenge proceeds (P<0.01). The percentage of Th17 cells derived from the cell suspension of the spleen gradually increased and it was positively correlated with the degree of asthmatic airway remodeling (P<0.01). The percentage of CD4+CD25+Treg cells from the suspension gradually decreased and it was negatively correlated with the degree of asthmatic airway remodeling (P<0.01). Conclusions In mice with asthma, as the challenge proceeds, the airway remodeling becomes more severe, the percentage of Th17 cells increases, and the percentage of CD4+CD25+Treg cells decreases. The immunological imbalance is possibly one of the important factors inducing airway remodeling.关键词
哮喘/气道重塑/TH17细胞/CD4+CD25+调节性T细胞/小鼠Key words
Asthma/Airway remodeling/Th17 cell/CD4+CD25+regulatory T cell/Mice引用本文复制引用
娄春艳,李敏,李丽..哮喘小鼠气道重塑过程中CD4+CD25+调节性T细胞和Th17细胞表达的动态变化[J].中国当代儿科杂志,2015,(9):994-1000,7.基金项目
四川省卫生厅科研项目（070089）。 （）
