中国临床药理学杂志Issue(15)：1526-1529,4.DOI:10.13699/j.cnki.1001-6821.2015.15.018
德都红花－7味散对基质金属蛋白酶及转化生长因子β1在肝纤维化中表达的影响
Influences of Deduhonghua -7 powder on matrix metalloproteinases and transforming growth factor beta expression in liver fibrosis
摘要
Abstract
Objective To observe the therapeutic effect of Deduhonghua-7 powder on liver fibrosis and its correlation with matrix metalloproteinases ( MMPs) and transforming growth factor β1 ( TGF-β1 ) .Methods The rats were divided into 4 groups, blank group, model group, control group, experiment group, 8 rats each group.The CCl4 was used to induce rat liver fibrosis.The control group was given colchicine pill, CMC sodium cellulosate was used to prepare 2% of colchicine suspen-sion, once time per day, 0.2 mL · kg-1 each time.The experiment group was given Deduhonghua-7 powder, CMC sodium cellulosate was used to prepare 3% of Deduhonghua-7 powder suspension, once time per day, 0.2 mL· kg -1 each time; blank group and model group were given same volume of pure water.All rats were sacrificed after 40 days of medication.Western blot was acted to detect the matrix metalloproteinase-1 (MMP-1), matrix metalloproteinase-13 (MMP-13),tissue inhibitor of metalloproteinases-1 (TIMP-1) protein expression.The PCR was used to detect the changesoftype Ⅰ, Ⅲ collagen, TGF -β1 mRNA gene expression.Results The MMP-1, MMP -13 protein expression in rats of model group was down -regulated compared with blank group ( P<0.05 ).The TIMP-1 protein expression was up-regulated compared with blank group ( P<0.05 ) . The mRNA expressions of typeⅠprocollagen, typeⅢprocollagen and TGF-β1 were increased compared with blank group(P<0.05).The MMP-1, MMP-13 protein expression of control group and experiment group were significant-ly up-regulated compared with model group.The TIMP-1 protein expression significantly down-regulated compared with model group.The mRNA gene expression of type I procollagen, typeⅢprocollagen and TGF-β1 were down-regulated compared with model group ( P<0.05 ).Conclusion Deduhonghua-7 powder regulate and maintain the stabilization of MMP/TIMP to block the expression of TGF-βand inhibit the transcription of type I,Ⅲ collagen and TGF-β1 mRNA to reach the purpose of detention of occurrence and development of liver fibrosis.关键词
德都红花-7味散/基质金属蛋白酶/转化生长因子β1/肝纤维化Key words
deduhonghua-7 powder/matrix metalloproteinases/transforming growth factorβ1/liver fibrosis分类
医药卫生引用本文复制引用
巴图德力根,徐艳华,韩志强,山丹,娜日苏,安达,塔娜..德都红花－7味散对基质金属蛋白酶及转化生长因子β1在肝纤维化中表达的影响[J].中国临床药理学杂志,2015,(15):1526-1529,4.基金项目
国家自然科学基金资助项目 （）
