中国全科医学Issue(27)：3314-3319,6.DOI:10.3969/j.issn.1007-9572.2015.27.012
藏雪莲多糖通过P38丝裂原激活蛋白激酶通路减轻中波紫外线辐射后皮肤角质形成细胞凋亡引起炎性损伤的研究
Alleviation of Inflammatory Damage Induced by the Apoptosis of HaCaT Cells After Ultraviolet B Radiation by Saussurea Tridactyla Sch. -Bip. polysaccharides Through p38MApK passage
摘要
Abstract
Objective To investigate whether Saussurea Tridactyla Sch. - Bip. ( SSB ) polysaccharides could alleviate inflammatory damage induced by the apoptosis of HaCaT cells after ultraviolet B( UVB) radiation through P38MAPK passage. Methods From October 2014 to March 2015,SSB polysaccharideswere extracted,and HaCaT cells were cultured in vitro and were respectively divided into low dose radiation group(30 mJ/cm2 ,radiation 1 h,group A),high dose radiation group (60 mJ/cm2,radiation 1 h,group B). Each of the two groups were further divided into control group(group 1),UVB radiation group(UVB,group 2),low dose SSB polysaccharide group(UVB+SSB 10 mg/ml,group 3),high dose SSB polysaccharide group(UVB+SSB 40 mg/ml,group 4). ELISA method was used to test and compare the levels of IL-6,TNF-α,P38MAPK, P53,Bcl-2,Bax and Caspase-3. Results Group A2 was higher(P<0. 05)in the levels of IL-6,TNF-α,P38MAPK, P53,Bax and Caspase-3 and lower(P<0. 05)in the level of Bcl-2 than group A1;group A3 was higher(P<0. 05)than group A1 in the levels of IL-6,TNF-α,P38MAPK and Caspase-3;group A4 was higher(P<0. 05)than group A1 in the levels of TNF-α and Bcl-2;group A3 and group A4 were lower(P<0. 05)in the levels of IL-6,TNF-α,P38MAPK, P53,Bax and Caspase-3 and higher(P<0. 05)in the level of Bcl-2 than group A2;group A4 was lower(P<0. 05)in the levels of IL-6,TNF-αand Caspase-3 and higher(P<0. 05)in the level of Bcl-2 than group A3. Group B2 was higher(P<0. 05)in the levels of IL-6,TNF-α,P38MAPK,P53,Bax and Caspase-3 and lower(P<0. 05)in the level of Bcl-2 than group B1;group B3 was higher(P<0. 05)in the levels of IL-6 and TNF-αand lower(P<0. 05)in the level of Bcl-2 than group B1;group B4 was higher(P<0. 05)in the levels of IL-6 and Bcl-2 and lower(P<0. 05)in the level of TNF-αand Caspase-3 than group B1;group B3 and group B4 were lower(P<0. 05)in the levels of IL-6,TNF-α,P38MAPK, P53,Bax and Caspase-3 and higher(P<0. 05)in the level of Bcl-2 than group B2;group B4 was lower(P<0. 05)in the levels of IL-6,TNF -α and Caspase -3 and higher(P <0. 05)in the level of Bcl-2 than group B3. Conclusion SSB polysaccharides could alleviate inflammatory damage induced by the apoptosis of HaCaT cells after UVB radiation through P38MAPK passage.关键词
紫外线/藏雪莲多糖/皮肤角质形成细胞/白细胞介素6/肿瘤坏死因子α/丝裂原激活蛋白激酶类/淋巴瘤,B细胞/Bcl-2相关X蛋白质/半胱氨酸蛋白酶类Key words
Ultraviolet rays/Saussurea Tridactyla Sch. - Bip. polysaccharides/HaCaT cells/Interleukin -6/Tumor necrosis factor - alpha/Mitogen - activated protein kinases/Lymphoma,B - cell/Bcl-2 - associated X protein/Cysteine proteases分类
医药卫生引用本文复制引用
郭砚,孙娟,王丽雯..藏雪莲多糖通过P38丝裂原激活蛋白激酶通路减轻中波紫外线辐射后皮肤角质形成细胞凋亡引起炎性损伤的研究[J].中国全科医学,2015,(27):3314-3319,6.基金项目
青海省（应用）基础研究计划项目 （）
