许宁 1平芬 2徐鑫 1诸葛铭宁 1张凤蕊 2韩书芝2
作者信息
- 1. 050051河北省石家庄市，河北省人民医院急诊科
- 2. 050051河北省石家庄市，河北省人民医院呼吸内二科
- 折叠
摘要
Abstract
Objective To investigate the pulmonary inflammatory injury in rats induced by acute exposure to PM2. 5 and to explore the antagonistic effect of low,medium,and high dose of Lianhuaqingwen to the inflammatory injury. Methods From June to December in 2013,selected 48 healthy adult Wistar rats. Using random number table method,divided them into control group,sodium chloride group,PM2. 5 group,low-dose group,medium-dose group and high-dose group,with 8 rats in each group. Airborne fine particulate matters were provided by Shijiazhuang Environmental Monitoring Center,and PM2. 5 suspension liquid was prepared. Control group was not given any intervention;sodium chloride group was given trachea instillation of 0. 9%sodium chloride solution( 1 ml/kg );PM2. 5 group was given trachea instillation of 1 ml/kg ( 7. 5 mg/kg ) PM2. 5 suspension liquid;low-dose,medium-dose and high -dose groups were respectively given gavage with 2 g/kg,4 g/kg and 8 g/kg Lianhuaqingwen solution of 10 ml/kg for 4 days,and the three groups were given trachea instillation of 1 ml/kg(7. 5 mg/kg) PM2. 5 suspension liquid on the fourth day and were killed 24 hours later. Pathological morphologic changes were observed under light microscope,and ELISA was employed to examine the levels of IL-1,IL-6 and TNF-αin BALF and serum. Results In control group and sodium chloride group, no abnormal manifestations in pulmonary tissue slices were observed under light microscope;in PM2. 5 group,exudation of inflammatory cells,interstitial proliferation of fibrous tissue and interstitial edema were noted in pulmonary tissue slices under light microscope;in the three Lianhuaqingwen groups, we found the exudation of inflammatory cells in alveolar space reduced with the increase of the dosage of Lianhuaqingwen. Sodium chloride group and control group were not significantly different in the levels of IL-1,IL-6 and TNF-α in BALF and serum(P>0. 05);PM2.5 group was higher than sodium chloride group in the level of IL-1 in BALF,and PM2. 5 group was higher than control group and sodium chloride group in the level of IL-1 in serum and the levels of IL-6 and TNF-αin BALF and serum(P<0. 05);high-dose group was lower than control group,sodium chloride group,PM2. 5 group,low-dose group and medium group in the level of IL-1 in BALF,lower than control group,sodium chloride group,PM2. 5 group and low-dose group in the level of IL-6 in BALF and serum,lower than PM2. 5 group,low-dose group and medium-dose group in the level of TNF-α in BALF and the level of IL-1 in serum, and lower than PM2. 5 group and low -dose group in the level of TNF -α in serum ( P <0. 05 ). Conclusion The acute exposure to PM2. 5 can induce pulmonary inflammatory injury in rats, Lianhuaqingwen could produce antagonistic effects to the inflammatory injury.关键词
肺炎/颗粒物/支气管肺泡灌洗液/白细胞介素/肿瘤坏死因子αKey words
Pneumonia/Particulate matter/Bronchoalveolar lavage fluid/Interleukin/Tumor necrosis factor-alpha分类
医药卫生
