中国医科大学学报2015，Vol.44Issue(8)：685-689,5.
低剂量静脉注射米诺环素后处理对大鼠缺血再灌注心肌的保护作用
Protective Effect of Intravenous Infusion of Low Dose Minocycline Postconditioning on Myocardial Ischemia-reperfusion Injury in Rat
摘要
Abstract
Objective To evaluate the effects of low dose intravenous minocycline postconditioning on myocardial ischemia-reperfusion injury in rat,and to investigate the possible mechanisms. Methods Forty-eight male wistar rats were randomly divided into four groups:sham-operation (SO)group,ischemia-reperfusion(IR)group,low-dose minocycline(3 mg/kg,LM)group and high-dose minocycline(10 mg/kg,HM)group. The rat model of myocardial IR was established by occlusion of the left anterior descending coronary artery for 45 minutes and reperfusion for 120 minutes.After the reperfusion,the parameters of haemodynamics were recorded;creatine kinase MB(CK-MB),cardiac troponin-I(cTn-I),malond-ialdehyde(MDA),superoxide dismutase(SOD)in serum and myocardium,myocardial apoptosis index(AI)and the myocardial tissue morphology were determined. Results Compared with IR group,LM and HM treatment significantly reduced the levels of CK-MB form,cTn-I,AI and MDA, lowered LVEDP,enhanced LVSP and ±dp/dtmax,elevated the activity of SOD in serum and myocardium(P<0.05). The effect of HM is stronger than LM on these above mentioned indicators,but the difference was not statistical significance(P>0.05). Conclusion LM postconditioning can protect against myocardial IR injury,and the protective effect may be related to the scavenging of oxide free radical,which further restrain the reac-tion of lipid peroxidation and apoptosis.关键词
米诺环素/缺血后处理/心肌缺血再灌注损伤Key words
minocycline/ischemia postconditioning/myocardial ischemia-reperfusion injury分类
医药卫生引用本文复制引用
张利群,陈咏君,崔仁善,齐国先..低剂量静脉注射米诺环素后处理对大鼠缺血再灌注心肌的保护作用[J].中国医科大学学报,2015,44(8):685-689,5.基金项目
辽宁省教育厅科学研究一般项目(L2013399) （L2013399）
沈阳医学院博士科研启动基金(20133050) （20133050）
