中国医院用药评价与分析2015，Vol.15Issue(8)：1034-1036,3.DOI:10.14009/j.issn.1672-2124.2015.08.017
高效液相色谱法测定感冒热毒清合剂中绿原酸的含量
Determination of the Content of Chlorogenic Acid in Ganmaoreduqing ( GMRDQ) Mistura by HPLC
邹晓华 1陈明洁 1方琅 1张扬2
作者信息
- 1. 解放军第117医院药剂科,浙江杭州 310013
- 2. 浙江省中医药研究院中药研究中心,浙江杭州 310007
- 折叠
摘要
Abstract
OBJECTIVE:To establish a method of HPLC to determine the content of chlorogenic acid in Ganmaoreduqing ( GMRDQ) mistura. METHODS:The content of chlorogenic acid in mistura was determined by HPLC with a SHISEIDO CAPCELL PAK MG-C18 column(4. 6 mm × 250 mm, 5 μm), acetonitrile-0. 4% phosphoric acid ( V:V=88:12) was used as mobile phase and the flow rate was 1. 0 ml/min. The column temperature was 40℃ and the wavelength of the detector was set at 327 nm. RESULTS:The linear range for chlorogenic acid was 5. 02μg/ml~100. 4μg/ml ( r =0. 999 5 ) . The average recovery for chlorogenic acid were 100. 37%, 103. 46%, 101. 20%. CONCLUSIONS:This thesis tries to establish a method of HPLC for determination of the content of chlorogenic acid in GMRDQ mistura for the first time, and the results are accurate with good repeatability,and the method can be used for quality assessment of GMRDQ mistura.关键词
高效液相色谱法/感冒热毒清合剂/绿原酸/含量Key words
HPLC/Ganmaoreduqing mistura/Chlorogenic acid/Content分类
医药卫生引用本文复制引用
邹晓华,陈明洁,方琅,张扬..高效液相色谱法测定感冒热毒清合剂中绿原酸的含量[J].中国医院用药评价与分析,2015,15(8):1034-1036,3.
