组织工程与重建外科杂志Issue(4)：225-228,233,5.DOI:10.3969/j.issn.1673-0364.2015.04.002
软骨细胞膜片复合3D打印内支撑构建管状软骨的初步研究
Construction of Tubulous Cartilage with Chondrocyte-sheets and 3D Printed Supporter
摘要
Abstract
Objective To construct tubulous cartilage by porcine auricular cartilage-derived chondrocyte-sheets combing with 3D printed supporter for repairing tracheal cartilage defects. Methods Primary chondrocytes were isolated from porcine auricular cartilage with methods of trypsin enzyme digestion and collagenase enzyme digestion. After proliferation in vitro, the chondrocytes of passage 2 were seeded on culture dish with a high density of 5 ×105 cells/mL, and intact chondrocyte-sheets were detached after cultured in a specific cell sheet culture environment for 14 days in vitro. Four detached chondrocyte-sheets were rolled onto a 3D printed supporter of PA material one by one to construct a tubulous cartilage-like complex. Twelve weeks’ static culture in vitro as well as 8 weeks' subcutaneous culture in nude mouse were performed. Gross inspection and paraffin section staining were applied for evaluation. Results Both in-vitro and in-vivo culture contributed to formation of tubulous cartilage with specific mechanical property. The cartilage extracellular matrix secretion and mechanical property of tissue engineered tubulous cartilage tissue cultured in vivo were better than that cultured in vitro. Conclusion It is feasible to construct tissue engineered tubulous cartilage tissue by chondrocyte-sheets and 3D printed PA supporter for tracheal cartilage repair.关键词
细胞膜片技术/软骨细胞膜片/3D打印支架/管状软骨Key words
Cell sheet technology/Chondrocyte sheets/3D printed supporter/Tubulous cartilage tissue分类
生物科学引用本文复制引用
葛阳,鲁亚南,韩露,冯蓓,白洁,何晓敏,殷猛,王伟,徐志伟,付炜..软骨细胞膜片复合3D打印内支撑构建管状软骨的初步研究[J].组织工程与重建外科杂志,2015,(4):225-228,233,5.基金项目
国家自然科学基金（31200735，81370117）；上海市自然科学基金-青年项目（12ZR1446500）；上海市浦东新区科技发展基金（PKJ2012-Y48）；上海交通大学医工交叉项目（YG2012MS36，YG2012MS35）；上海卫生和计划生育委员会（20144Y0166）；上海高校选拔培养优秀青年教师基金（ZZjdyx1211）。 （）
