安徽医科大学学报2015，Vol.50Issue(9)：1219-1222,4.
兴奋下丘脑腹外侧视前区对结节乳头体核c-Fos表达的影响及其受体途径
The effect of c-Fos expression in tuberomammillary after exciting ventrolateral preoptic and its receptor passway
摘要
Abstract
Objective To investigate the effect of c-Fos expression in tuberomammillary nucleus ( TMN) after ex-citing ventrolateral preoptic ( VLPO) and its receptor passway. Methods The expressions of mGluR5 in VLPO and GABAA Rβ1 in TMN were determined by immunostaining. L-Glutamate ( L-Glu) was microinjected into VLPO with stereotaxic technique to observe the changes of c-Fos expression in TMN. Results Positive protein of mGluR5 could be identified by Anti-mGluR5 in VLPO and also positive protein of GABAAR was identified in TMN by Anti-GABAARβ1. c-Fos expression in TMN reduced after microinjecting L-Glu in VLPO compared with the control group. The difference between them was significant (P<0. 01). Conclusion The metabotropic glutamate recep-tor in VLPO and GABA receptor in TMN mediate the regulation of VLPO-TMN passway. VLPO inhibits the TMN neuronal activity via GABAAR.关键词
下丘脑腹外侧视前区/结节乳头体核/c-Fos/免疫组织化学Key words
ventrolateral preoptic/tuberomammillary nucleus/c-Fos/immunohistochemistry分类
医药卫生引用本文复制引用
丁睿,王媛,丁正霞,吴芳,许奇,张瑾,王烈成..兴奋下丘脑腹外侧视前区对结节乳头体核c-Fos表达的影响及其受体途径[J].安徽医科大学学报,2015,50(9):1219-1222,4.基金项目
国家自然科学基金(编号:81071075、81401100) （编号:81071075、81401100）
安徽高校 省级自然科学研究项目(编号:KJ2014A116) （编号:KJ2014A116）
