安徽医科大学学报2015，Vol.50Issue(9)：1223-1228,6.
自噬与脂联素诱导的人乳腺癌MCF-7细胞凋亡的关系
The relation of autophagy and apoptosis induced by adiponectin in the MCF-7 cells
摘要
Abstract
Objective To explore the effect of adiponectin(Acrp30) on the proliferation, migration, autophagy, apoptosis of MCF-7 cells and investigate the relation of autophagy and apoptosis induced by adiponectin. Methods Divided the MCF-7 cells into control group ( no Acrp30 added ) , drug added group, then cultured for some hours . The cells viability was detected by methyl thiazolyl tetrazolium ( MTT ) assay . An appropriate concentration Acrp30(100 ng/ml) was selected to add into the cells after culturing for some hours, the morphological changes, migration and autophagy level were quantified by invert microscope, wound-healing assay and Western blot respec-tively. The total apoptotic rate of added Acrp30 and pretreated with an autophagy inhibitor 3-methyladenine aggra-vated ( MA) groups were analyzed by flow cytometry. Results ①Compared with the control group,the viability of 50,100 ng/ml cultured 72 h and 200 ng/ml cultured 48,72 h groups were lower(P<0. 05);②Compared with the control group,the migration lengths of Acrp30 added group 48,72 h were decreased (P<0. 01);③ The level of autophagy ( autophagy associated protein LC3 B-Ⅱ/LC3 B-Ⅰ) was up-regulated after Acrp30 added group cultured 24,48 h compared with the control group(P<0. 05,P<0. 01), but the ratio was reduced along with time,after 72 h,it had no difference with the control;④The apoptotic rate of MCF-7 cells increased after 48 h,72 h treated with 100 ng/ml Acrp30 and pretreated with MA groups (P<0. 05,P<0. 01) compared with the control group. Further-more, the 72 h apoptotic rate of (Acrp30+MA) group was increased compared with Acrp30 group (P<0. 05). Conclusion Acrp30 can inhibit the growth and migration of MCF-7 cells and induce the autophagy and apoptosis. Inhibit autophagy can promote the apoptosis which induced by adiponectin in MCF-7 cells.关键词
脂联素/MCF-7细胞/增殖/自噬/凋亡Key words
adiponectin/MCF-7 cell/proliferation/autophagy/apoptosis分类
医药卫生引用本文复制引用
刘佳,王佑民,胡红琳,冯双双..自噬与脂联素诱导的人乳腺癌MCF-7细胞凋亡的关系[J].安徽医科大学学报,2015,50(9):1223-1228,6.基金项目
安徽省科技攻关项目(编号:1206c0805034) （编号:1206c0805034）
中华医学会临床医学科研专项基金项目(编号:13040420427) （编号:13040420427）
