安徽医科大学学报2015，Vol.50Issue(9)：1237-1242,6.
选择性Bcl-2抑制剂ABT-199对乳腺癌细胞MDA-MB-231的放疗增敏作用
Radiosensitization of breast cancer cells MDA-MB-231 with the selective Bcl-2 inhibitor ABT-199
摘要
Abstract
Objective To study the radiosensitization effect of selective Bcl-2 inhibitor, ABT-199 in breast cancer cells MDA-MB-231 and to explore its possible mechanism. Methods The MDA-MB-231 cells in logarithmic phase were divided into control group, drug group, radiation group and combination group (radiation+ABT-199). The anti-proliferative effects of radiation and ABT-199 in MDA-MB-231 were measured respectively with MTT assay and the value of IC20 was calculated. The cell cycle arresting and apoptosis of MDA-MB-231 cells were measured by flow cytometry. Caspase-3 activity was assayed by caspase activity assay kit. The Bcl-2, Bax, Bcl-xl expression were detected by western blot. Results ABT-199 inhibited the proliferation of MDA-MB-231 in a time-dependent and dose-dependent manner. With the increasing of ABT-199 concentrations, MDA-MB-231 cells were blocked in G0/G1 phase. The apoptosis rate in combination group was significantly higher than radiation group ( 16. 9% vs 84. 5%) . Compared with the radiation group, Bcl-2 expression of the combination group were significantly reduced ( P<0. 05) and Bcl-xl expression showed no significant change, while its Bax expression and Caspase-3 activity in-creased ( P<0. 05 ) . Conclusion Bcl-2 selective inhibitor ABT-199 can effectively inhibit the proliferation of hu-man breast cancer cell line MDA-MB-231 and induce cell G0/G1 phase arrest and apoptosis. ABT-199 can signifi-cantly improve the radiosensitivity of MDA-MB-231 .关键词
MDA-MB-231/ABT-199/Bcl-2抑制剂/放疗/协同效应Key words
MDA-MB-231/ABT-199/Bcl-2 inhibitor/radiotherapy/synergistic effect分类
医药卫生引用本文复制引用
钱振珏,牛伶,鲍扬漪,刘柳,童斯浩,龚理,胡长路..选择性Bcl-2抑制剂ABT-199对乳腺癌细胞MDA-MB-231的放疗增敏作用[J].安徽医科大学学报,2015,50(9):1237-1242,6.基金项目
安徽省科技攻关项目(编号:12010402123) （编号:12010402123）
