安徽医科大学学报2015，Vol.50Issue(9)：1252-1258,7.
呼吸道合胞病毒合并肺炎克雷伯菌感染所致肺炎与TLR4-NF-κB信号通路关系的初步研究
A preliminary study on the relations between pneumonia induced by RSV combined with Klebsiella pneumonia infection and TLR4-NF-κB signal transduction pathway
摘要
Abstract
Objective This study investigates SD rats infected with pneumonia respiratory syncytial virus ( RSV) combined with klebsiella pneumonia ( Kp ) to explore its relationship with TLR4-NF-κB signal transduction path-way. Methods A total of 60 SD rats were divided into four groups randomly:control group, RSV group, Kp group and Kp + RSV group. All animals were infected with RSV or Kp using intranasal method. We observed the weights, lung index, histopathological changes in the lung tissues by HE staining, the expression of TLR4, NF-κB mRNA with RT-PCR, NF-κB protein by Western blot analysis in the lung tissues and the concentrations of TNF-α, IL-1β with ELISA at 0,1,3,5,7 days after RSV or Kp post-infections. Results Compared with control group,dif-fuse thickening of alveolar interval and inflammatory cells infiltration were observed in RSV and Kp group,and with the extension of infection time, the infection alveolar structure increased gradually. The expression of LI,TLR4 mR-NA,NF-kB mRNA,NF-kB protien,TNF-α and IL-1β increased and the relationship between RSV and Kp showed time dependence;in RSV + Kp group , the expression of LI, TLR4 mRNA, NF-kB mRNA, NF-kB protein, TNF-αand IL-1β increased gradully and lung tissue inflammation increased significantly. Conclusion RSV combined with Kp infection has synergistic effects and aggravates pulmonary inflammation. RSV combined with Kp infection can induce pulmonary defence cells release cytokines TNF-α and IL-1β by TLR4-NF-κB signal transduction path-way.关键词
呼吸道合胞病毒/肺炎克雷伯菌/肺炎/TLR4/NF-κB/炎性因子Key words
respiratory syncytial virus/Klebsiella pneumonia/pneumonia/TLR4/NF-κB/inflammatory cytokines分类
医药卫生引用本文复制引用
王云,黄升海,吴璇,李振兴,于莉,汪旻旻..呼吸道合胞病毒合并肺炎克雷伯菌感染所致肺炎与TLR4-NF-κB信号通路关系的初步研究[J].安徽医科大学学报,2015,50(9):1252-1258,7.基金项目
安徽省自然科学基金项目(编号:1308085MH129) （编号:1308085MH129）
安徽高校省级自然科学研究重点项目(编号:KJ2012A152) （编号:KJ2012A152）
