安徽医科大学学报2015，Vol.50Issue(9)：1258-1261,4.
miR-139在结直肠癌组织中的表达及其对癌细胞生长的影响
Expression of miR-139 and its role in colorectal cancer cells
摘要
Abstract
Objective To explore the expression of miR-139 in colorectal cancer and study its role in colorectal cancer HT29 cells in vitro. Methods Real-Time PCR was used to analyze the expression of miR-139 in 20 color-ectal cancer tissues and their matched adjacent tissues. HT29 cells were transfected with a miR-139 mimics, then MTT and Softagar methods were used to detect the proliferation of the cells. The migration and invasion of HT29 cells were investigated using Transwell assay. Results The expression of miR-139 was down-regulated in colorectal cancer tissues compared with those in adjacent tissues (P<0. 01). According to MTT method, from the first day to fifth day,the OD of HT29 cells transfected with miR-139 mimics significantly reduced compared with the control group. According to Softagar method,the control group counts(41 ± 5)each field compared with miR-139(72 ± 5) each field(P<0. 01). According to Transwell assay,compared with the control group,transfection of miR-139 mim-ics inhibited the migration and invasion of HT29 cells. Conclusion The expression level of miR-139 is lower in colorectal cancer. Transfection of HT29 cells with a miR-139 mimics can inhibit the cell proliferation,migration and invasion in vitro,suggesting that miR-139 is a potent tumor suppressor.关键词
microRNA/miR-139/结直肠肿瘤/肿瘤抑制Key words
microRNA/miR-139/colorectal carcinoma/tumour suppressor分类
医药卫生引用本文复制引用
龙腾云,余昌俊,张敏,余康敏,朱正杰,欧阳欢..miR-139在结直肠癌组织中的表达及其对癌细胞生长的影响[J].安徽医科大学学报,2015,50(9):1258-1261,4.基金项目
国家自然科学基金(编号:31201022) （编号:31201022）
