安徽医科大学学报2015，Vol.50Issue(9)：1297-1300,4.
糖皮质激素对重症肌无力患者外周血调节性T细胞中Foxp3及其胞内CTLA-4表达的影响
Expression alteration of Foxp3 and intracellular CTLA-4 of regulatory T cells in peripheral blood of myasthenia gravis patients under glucocorticoids treatment
摘要
Abstract
Objective To study the expression alteration of Foxp3 and intracellular CTLA-4 of CD4 +CD25 +regula-tory T cells in peripheral blood of myasthenia gravis patients under glucocorticoids treatment. Methods 34 patients with myasthenia gravis were selected, and subgrouped with no treatment group(8 cases) or glucocorticoids treat-ment group(26 cases) . Another 20 healthy subjects were set as the control group. 6-color flow cytometry was used to analyze the expression of Foxp3 and intracellular CTLA-4 of CD4 +CD25 + regulatory T cells in their peripheral blood of participants. Results ①Compared with the control group, the level of Foxp3 and intracellular CTLA-4 of CD4 +CD25 +regulatory T cells in peripheral blood of in no treatment group were significantly decreased ( P <0. 05 ) . ② the level of Foxp3 and intracellular CTLA-4 of CD4 +CD25 +regulatory T cells in peripheral blood of pa-tients with glucocorticoids treatment group were significantly higher than that of no treatment group(P<0. 05), but were still lower than that of the control group(P<0. 05). ③Foxp3 expression was positively correlated with intra-cellular CTLA-4 expression in CD4 +CD25 +regulatory T cells of patients with myasthenia gravis ( r=0. 870 , P<0. 001 ) . Conclusion Low expression of Foxp3 and intracellular CTLA-4 of CD4 +CD25 + regulatory T cells in pe-ripheral blood is present in patients with myasthenia gravis, glucocorticoids could significantly increase the expres-sion of Foxp3 and intracellular CTLA-4, however, which still do not reach the immune state of healthy subjects.关键词
重症肌无力/CD4+CD25+Treg/Foxp3/CTLA-4/糖皮质激素Key words
myasthenia gravis/CD4 +CD25 + Treg/Foxp3/CTLA-4/glucocorticoids分类
医药卫生引用本文复制引用
庄战强,许文华,吴元波,张翠萍,李庆,任明山..糖皮质激素对重症肌无力患者外周血调节性T细胞中Foxp3及其胞内CTLA-4表达的影响[J].安徽医科大学学报,2015,50(9):1297-1300,4.基金项目
安徽省青年科学基金项目(编号:11040606Q08) （编号:11040606Q08）
