安徽医科大学学报2015，Vol.50Issue(10)：1377-1380,4.
人E2F1蛋白的表达及与Sedlin蛋白共定位研究
The expression of human E2F1 and the co-localization with Sedlin
摘要
Abstract
Objective To investigate the expression and localization of E2F1 in eukaryotic cells and the co-locali-zation with Sedlin. Methods To construct eukaryotic expression plasmid pcDNA3. 1-E2F1-FLAG. The plsamid was transfected into HEK 293T cells and the expression was checked by Western blot. Its expression in COS7 cells was detected by fluorescence microscopy. Results The eukaryotic expression plasmid of pcDNA3. 1-E2F1-FLAG was constructed successfully, which could be effectively expressed in HEK 293T and COS7 cells. E2F1 was pre-dominantly distributed in the nucleus, and it co-localized in the nucleus with Sedlin. Conclusion E2F1 can over-express efficiently in HEK 293T and COS7 cells, and it can colocalize with Seldin, which provides important bases for further study on E2F1 including its functions and associated proteins.关键词
E2F1/转染/基因表达/细胞定位Key words
E2F1/transfection/gene expression/cellular localization分类
医药卫生引用本文复制引用
汪燕燕,潘林鑫,王梦媛,刘晓颖,范礼斌..人E2F1蛋白的表达及与Sedlin蛋白共定位研究[J].安徽医科大学学报,2015,50(10):1377-1380,4.基金项目
国家自然科学基金青年基金(编号:81201368 ) （编号:81201368 ）
安徽省级大学生创新创业训练计划项目(编号:AH201410366017, AH201410366132) （编号:AH201410366017, AH201410366132）
