安徽医科大学学报2015，Vol.50Issue(10)：1385-1389,5.
大鼠非酒精性脂肪肝病程中细胞色素P450酶的分析
Analysis of hepatic cytochrome P450 enzymes in rats with progressive stages of non-alcoholic fatty liver disease
摘要
Abstract
Objective To study the alterations of cytochrome P450 enzymes in rats liver with progressive stages of non-alcoholic fatty liver disease ( NAFLD) . Methods NAFLD models were established. Activities of hepatic as-partate aminotransferase ( AST) , alanine aminotransferase ( ALT) and levels of triglyceride ( TG) were measured by assay kit. The CYP1A2, CYP2D6, CYP2E1 and CYP3A1 protein expressions were detected by Western blot a-nalysis. Results Higher activities of ALT and AST were detected(P<0. 01)as the severity of NAFLD increased. TG levels increased remarkably in the range from normal to serious steatosis, and then decreased dramatically. Pro-tein expressions of CYP1 A2 , CYP2 D6 and CYP3 A1 tended to decrease with NAFLD progression while CYP2 E1 was increased consecutively. Conclusion These results suggest that significant and novel changes occur in hepatic P450 expression during progressive stages of NAFLD. It may provide a valuable framework for physicians when de-termining the pharmacotherapeutic options and dosing regimens to patients with this disease.关键词
非酒精性脂肪肝/细胞色素P450酶/肝匀浆/蛋白表达Key words
non-alcoholic fatty liver disease/cytochrome P450 enzyme/liver homogenate/protein expression分类
医药卫生引用本文复制引用
余庆,许曼,赵倩茹,林元节,陈伟,金涌..大鼠非酒精性脂肪肝病程中细胞色素P450酶的分析[J].安徽医科大学学报,2015,50(10):1385-1389,5.基金项目
安徽省高等学校省级自然科学研究重点项目(编号:KJ2013A153) （编号:KJ2013A153）
