安徽医科大学学报2015，Vol.50Issue(10)：1399-1403,5.
microRNA-21对裸鼠人胆管癌细胞移植瘤EMT的影响
Effect of microRNA-21 on EMT of human cholangiocarcinoma xenografts in nude mice
摘要
Abstract
Objective To observe the effects of microRNA-21 ( miR-21 ) on the epithelial-mesenchymal transition ( EMT) of human cholangiocarcinoma xenografts in nude mice. Methods QBC-939 and QBC939-miR-21 cholan-giocarcinoma cells were subcutaneously injected into two groups of nude mice respectively as xenografts, expressions of E-cadherin, N-cadherin and Vimentin were detected by Western blot ,RT-PCR and immunohistochemical analy-sis. Results Compared with group QBC939,group QBC939-miR-21 xenografts showed the greater tumor weight and rapider growth,expression of E-cadherin decreased,whereas the protein expressions of Vimentin and N-cadherin increased. Conclusion miR-21 overexpression enhances tumor growth, which promotes EMT phenotype and the foundation for further study of miR-21 function in cholangiocarcinoma and mechanism is established.关键词
胆管癌/microRNA-21/上皮间质转化/移植瘤Key words
cholangiocarcinoma/microRNA-21/EMT/xenograft分类
医药卫生引用本文复制引用
晋志远,黄强,刘臣海,刘振,林先盛,谢放..microRNA-21对裸鼠人胆管癌细胞移植瘤EMT的影响[J].安徽医科大学学报,2015,50(10):1399-1403,5.基金项目
国家自然科学基金(编号:81272397 ) （编号:81272397 ）
安徽省自然科学基金(编号:1208085MH176) （编号:1208085MH176）
