安徽医科大学学报2015，Vol.50Issue(10)：1413-1417,5.
LCE3C在皮肤癌组织中分布和细胞定位及其调控机制的初步研究
The LCE3 C expression in human skin cancers and the potential regulation mechanisms
摘要
Abstract
Objective To investigate the LCE3C expresson in squamous cell carcinoma (SCC) and basal cell car-cinoma (BCC), and the potential mechanisms for regulating LCE3C expression. Methods Immunohistochemical method was performed to detect the expression levels of LCE3 C in the normal skin tissues or the lesion skin tissues from patients with SCC and BCC. To find the cellular localization of the LCE3C proteins, human skin cancer cells were transfected by recombinant plasmid ( pLCE3C-GFP) containing human LCE3C cDNA fused with the GFP, and then were observed under the confocal laser scanning microscopy. To further investigate the potential mecha-nisms for regulating LCE3C expression in skin cancer, Western blot was used to detect the expression levels of GRHL3 and LCE3C in the skin cancer cells. Results Immunohistochemical analysis showed that the LCE3C high-ly expressed at the stratum corneum in the lesion skin tissues from patients with SCC and BCC, compared with the normal skin tissues. LCE3C proteins fused with GFP in A431 cells were mainly observed in extracellular matrix ( ECM) based on the confocal laser scanning. Western blot analysis showed that the expression level of GRHL3 in the A431 cells was reduced than that of the HaCaT cells; on the contrary, the expression level of LCE3C signifi-cantly increased in the A431 cells. Furthermore, LCE3C expression was also significantly decreased in the GRHL3 over-expressed cell (A431/GRHL3) compared with the A431 cells. Conclusion Compared to the normal skin tis-sues, LCE3C is dramatically expressed at the corneous layers in the skin cancer tissues. LCE3C proteins are mainly expressed in ECM, and might be negatively regulated by GRHL3 in A431 cells.关键词
LCE3C/皮肤鳞状细胞癌/基底细胞癌/细胞定位Key words
LCE3 C/squamous cell carcinoma/basal cell carcinoma/cellular localization分类
医药卫生引用本文复制引用
陈琼琼,涂珍珍,王瑞,张思平,赵盼,成芳,查晓军,周海胜..LCE3C在皮肤癌组织中分布和细胞定位及其调控机制的初步研究[J].安徽医科大学学报,2015,50(10):1413-1417,5.基金项目
国家自然科学基金(编号:81172591) （编号:81172591）
