安徽医科大学学报2015，Vol.50Issue(10)：1422-1425,4.
M3 受体激动剂对慢性心衰大鼠心肌ICa-L及[Ca2+]i的影响
Effect of M3 R agonist on ICa-L and [Ca2+]i in myocardium of chronic heart failure rats
摘要
Abstract
Objective To investigate the protective effects of choline on ICa-L and [Ca2+]i in myocardium from rats with chronic heart failure. Methods The single ventricular myocytes were isolated by use of collegenase type II, ICa-L was recorded by whole-cell patch clamp technique;[Ca2+]i was detected by laser scanning confocal micro-scope in ventricular myocytes. Results The normalized peak currents of ICa-L in ventricular myocytes were elevated by choline group compared with those in CHF group, which could be neutralized by U73122 and partly reverted by 4-DAMP. The [Ca2+]i induced by KCl in ventricular myocytes were significantly increased by choline group, which could be suppressed by U73122 and also partly reverted by 4-DAMP. Conclusions The possible mechanism of protection of M3 receptor involved in the protection of CHF rats is due to the open L type calcium channel,increased [Ca2+]i via activation of Gq/11-PLC signal passway.关键词
胆碱/U73122/4-DAMP/L-型钙电流/膜片钳/激光扫描共聚焦Key words
choline/U73122/4-DAMP/ICa-L/patch-clamp technique/laser scanning confocal microscope分类
医药卫生引用本文复制引用
栾海蓉,孙健,王得利,吴红,董琦..M3 受体激动剂对慢性心衰大鼠心肌ICa-L及[Ca2+]i的影响[J].安徽医科大学学报,2015,50(10):1422-1425,4.基金项目
国家自然科学基金青年基金项目(编号:81300163 ) （编号:81300163 ）
黑龙江省教育厅科研课题(编号:12531731) （编号:12531731）
