安徽医科大学学报2015，Vol.50Issue(10)：1443-1447,5.
镉诱导小鼠急性肝损伤模型的研究
Establishment of mouse model of acute liver injury induced by cadmium chloride
摘要
Abstract
Objective To establish the mouse model of acute liver damage induced cadmium chloride and elucidate the mechanism of Cd-induced-liver injury. Methods The adult male mice were intraperitoneally ( i. p. ) injected with a single dose of CdCl2 (4 mg/kg) and killed at different time(6, 12, 18, 24 h) after Cd treatment, or admin-istrated with different doses of CdCl2 (1, 2 or 4 mg/kg) i. p. and sacrificed at 18 h after Cd exposure. The control group received only equal volumes of normal saline. Histopathology of liver tissues, serum of ALT, AST and MDA, and MDA,NO,GSH,GSH-PX of liver tissues were observed. Results 18 hours after the treatment of using 4 mg/kg dosage, i. p, liver tissues appeared visible pathological changes with severe ballooning degeneration and necro-sis, the levels of ALT and AST in serum were obviously higher. In addition, at 18 h after Cd treatment, the levels of MDA and NO in liver tissues significantly increased in the cadmium group, however, the level of GSH and the activity of GSH-PX in liver tissues significantly decreased. Conclusion A mouse model of acute liver injury is successfully established by intraperitoneal injection with 4 mg/kg CdCl2 at 18 hours after Cd treatment. The mecha-nism might be associated with the increase of MDA and NO and the decrease of the level of GSH and the activity of GSH-PX in liver tissues.关键词
氯化镉/急性肝损伤/动物模型/MDA/NO/GSHKey words
cadmium chloride/acute liver injury/animal model/MDA/NO/GSH分类
医药卫生引用本文复制引用
吴永琴,关存杰,刘洪茂,姬艳丽,徐德祥..镉诱导小鼠急性肝损伤模型的研究[J].安徽医科大学学报,2015,50(10):1443-1447,5.基金项目
国家自然科学基金(编号:31000664 ) （编号:31000664 ）
安徽医科大学2011年博士科研资助项目(编号:XJ201114 ) （编号:XJ201114 ）
安徽医科大学"青年拔尖人才支持计划",高等学校省级特色专业建设点项目(编号:2013tszy010) （编号:2013tszy010）
