安徽医科大学学报2015，Vol.50Issue(10)：1497-1500,4.
吲哚胺2,3-二氧酶(IDO)与CINⅢ及宫颈癌T淋巴细胞的相关性研究
Correlation study between indoleamine 2,3-dioxygenase(IDO) and T lymphocytes in cervical cancer and CINⅢ
杜世华 1胡传玉 1鲁大伟 1李燕 1桑琳1
作者信息
- 1. 安徽医科大学附属合肥医院妇产科,合肥 230011
- 折叠
摘要
Abstract
Objective To investigate the expression of indoleamine2,3-dioxygenase(IDO) how to effect on T lym-phocyte activity, in order to understand the mechanism of immune escape of cervical cancer. Methods Selected 20 patients with CINⅢ,20 cases with cervical cancer and 20 cases with normal cervical for comparison, used flow cytometry to detcet the number of each group CD3 +T, CD4 +T, CD8 +T, CD4 +CD25 + Foxp3 +Tregs and CD4 +/CD8 +. Used semi-quantitative RT-PCR and Western blot to detect IDO mRNA and expression of IDO, and to ana-lyze the effection on T lymphocyte active in cervical tissue. Results The expression of IDO was significantly differ-ent among the three group(P<0. 001). The positive rate of IDO in cerival cancer was significantly higher than that in CINIII and normal cerival(P<0. 001). The positive rate of IDO in CINⅢ was significantly higher than that in normal cerival(P<0. 001). The number of CD3 +T, CD4 +T, CD8 +T and CD4 +CD25 +Foxp3 +Tregs was signifi-cantly different among the three group(P<0. 001). The positive expression of IDO was negatively correlated with the number of CD3 +T, CD4 +T, CD8 +T,and positively correlated with CD4 +CD25 +Foxp3 +Tregs. Conclusion IDO inhibits T lymphocyte activity. Cervical cancer patients with low immune function can cause the tumor cells es-cape form immune surveillance,may lead to disease progression.关键词
吲哚胺2,3-二氧酶/子宫颈癌/CINⅢ/T淋巴细胞Key words
indoleamine 2/3-dioxygenase/cervical cancer/CINⅢ/T lymphocytes分类
医药卫生引用本文复制引用
杜世华,胡传玉,鲁大伟,李燕,桑琳..吲哚胺2,3-二氧酶(IDO)与CINⅢ及宫颈癌T淋巴细胞的相关性研究[J].安徽医科大学学报,2015,50(10):1497-1500,4.
