安徽医科大学学报Issue(11)：1560-1565,6.
TLR7在呼吸道合胞病毒感染A549细胞诱导I型干扰素中的作用
The role of TLR7 in A549 cells with the respiratory syncytial virus infection induced type I interferon
摘要
Abstract
Objective This experiment was designed to explore the antiviral effect of TLR7 when A549 cells were infected by respiratory syncytial virus (RSV)to induce the production of type I interferon (IFN).Methods This experiment was divided into normal control group,RSV infection group and TLR7 silence group.In each group cell and culture supernatant were collected after having been infected for 4,8,12 and 24 h.TLR7 siRNA was trans-fected in A549 cells by transient transfection.With the use of the RT-PCR assay,TLR7 gene silencing effect could be detected;with the use of Trizol reagent,the total RNA could be extracted and the expression dynamics of mRNA in TLR7,IRF7,IFN-αand IFN-βcould be detected;with the use of Western blot,protein expression could be tested;with the use of ELISA,the variation of IFNα/βin the culture supernatant of A549 cells infected in the dif-ferent points of time could be detected.Results ① 24 h after transfection,TLR7 siRNA-2 could restrain the ex-pression of TLR7 mRNA effectively with statistical significance.② The expression of mRNA in TLR7,IRF7 and IFN-α/β,together with TLR7 protein,which existed time dependent relation with RSV infection,had increased af-ter A549 cells had been infected by RSV.Meanwhile,compared with RSV infection group,the expression of mR-NA in TLR7,IRF7 and IFN-α/β,together with TLR7 protein had decreased obviously in TLR7 silence group.③Compared with RSV infection group,the expression of IFN-α/βin cell culture supernatant and in TLR7 silent group had decreased,and the expression of IFN-αdecreased obviously.It went without saying that the difference of decrease in two groups had statistical significance.Conclusion After cell A549 have been infected by RSV, TLR7 could be activated and then induce type I interferon after having been infected by RSV,which play the role in anti-virus.Noticeably,IFN-αaccounts for the most of e type I interferon.关键词
呼吸道合胞病毒/A549细胞/Toll样受体7/siRNA/I型干扰素Key words
respiratory syncytial virus,A549 cells/Toll-like receptors 7/siRNA/Type I interferon分类
医药卫生引用本文复制引用
何汉文,王葱,杜博易,吴璇,于莉,汪,黄升海..TLR7在呼吸道合胞病毒感染A549细胞诱导I型干扰素中的作用[J].安徽医科大学学报,2015,(11):1560-1565,6.基金项目
安徽高校省级自然科学研究重点项目（编号KJ2012A152）；安徽省自然科学基金（编号1308085MH129）；安徽省级大学生创新创业训练计划项目 （）
