安徽医科大学学报Issue(11)：1588-1592,1593,6.
抗霉素A诱导血小板凋亡的分子机制研究
Study of molecular mechanisms on platelet apoptosis induced by antimycin A
摘要
Abstract
Objective To investigate the effects and molecular mechanism of platelet apoptosis induced by Antimy-cin A (AMA).Methods Washed healthy volunteers platelets were pre-incubated with different concentration AMA,and then depolarization of mitochondrial membrane potential (ΔΨm),phosphatidylserine (PS)externaliza-tion assay,the surface expression of P-selectin,the activation of integrin αIIβ3 and the production of intracellular reactive oxygen species (ROS),mitochondrial ROS were detected by flow cytometry.The activation of Caspase-3 was analyzed by Western blot.In inhibition experiment,washed platelets were pre-incubated mitochondrial ROS targeted antagonists Mito-TEMPO,and then stimulated with different concentration AMA.The apoptosis associated indicators were detected by flow cytometry.Results AMA dose-dependently induces depolarization of ΔΨm,PS exposure,Caspase-3 activation,and the production of intracellular ROS and mitochondrial ROS,but AMA does not induce platelet activation.Mito-TEMPO effectively reduces the depolarization of ΔΨm,PS exposure and the pro-duction of mitochondrial ROS which induced by the AMA.Conclusion AMA can induce platelet apoptosis,and mitochondria ROS may play an important role in the platelets apoptosis induced by the AMA.关键词
血小板/凋亡/抗霉素A/线粒体/活性氧Key words
platelets/apoptosis/antimycin A/mitochondria/reactive oxygen species分类
医药卫生引用本文复制引用
胡萍,朱永明,谢如锋,王志成..抗霉素A诱导血小板凋亡的分子机制研究[J].安徽医科大学学报,2015,(11):1588-1592,1593,6.基金项目
国家自然科学基金（编号81270650）；上海市自然科学基金资助项目 （）
