安徽医科大学学报Issue(11)：1606-1609,1610,5.
PICK1在肝纤维化中的表达变化及功能研究
The expression and effect of PICK1 in liver fibrosis
摘要
Abstract
Objective To explore the expression of protein interacting with Ca-kinase-1(PICK1)in fibrotic liver of mice and activated hepatic stellate cell,and investigate the effect of PICK1 on the activation of hepatic stellate cell strain (LX-2).Methods Liver fibrosis model in mice was induced by CCl4 and then the PICK1 level was detec-ted in fibrotic liver tissue.LX-2 cells were activated by transforming growth factor β1 (TGF-β1),and the protein level of PICK1 was detected by Western blot.After transfected with the plasmid expressing PICK1,LX-2 was stim-ulated with TGF-β1,the levels of α-smooth muscle actin(α-SMA),collagen type I (Col1a1 ),Smad2,3 and phosphorylation level of them were determined by Western blot.Results PICK1 was highly expressed in normal liver tissues and progressively down-regulated as liver fibrosis progressed.The expression of PICK1 was down-regu-lated in activated LX-2 induced by TGF-β1.The hepatic stellate cell transfected with PICK1-plasmid showed re-markablely decreased TGF-β1-induced α-SMA and Col1a1 expression and obviously declined phosphorylation levels of Smad2 and Smad3.Conclusion The expression of PICK1 decreases in fibrotic livers and activated hepatic stel-late cell.Over-expression of PICK1 can suppress the activation of hepatic stellate cell induced by TGF-β1,and probably because of the inhibitory effect on TGF-β/Smad pathway,which provides new ideas and targets for the prevention and treatment of liver fibrosis.关键词
PICK1/肝纤维化/肝星状细胞Key words
PICK1/Liver Fibrosis/hepatic stellate cell分类
医药卫生引用本文复制引用
陈培杰,李俊,黄成,孟晓明,蔡双朋..PICK1在肝纤维化中的表达变化及功能研究[J].安徽医科大学学报,2015,(11):1606-1609,1610,5.基金项目
国家自然科学基金（编号81273526、81473268）；安徽省重点科技攻关项目（编号1301042212）；安徽省自然科学基金（编号1308085MH145）；高等学校博士学科点专项科研基金 （）
