北京中医药大学学报Issue(9)：624-627,4.DOI:10.3969/j.issn.1006-2157.2015.09.010
在体单向肠灌流模型研究千金子甾醇在大鼠肠吸收特性
Characteristic of intestinal absorption of euphorbia factor L1 by rats single pass intestinal perfusion moder in situ
摘要
Abstract
Objective To study the characteristic of absorption of euphorbia factor L1 in intestine of rats, and to observe the effects of P-glycoprotein(P-gp)and multidrug resistance-associated protein(MRP2)on intestinal absorption of euphorbia factor L1 .Methods The contents of euphorbia factor L1 of intestinal perfusion fluid of duodenum, jejunum, ileum and colon in the rats in situ single-pass intestinal perfusion model were determined by using HPLC.The drug absorption rate constant( Ka ) and the apparent absorp-tion coefficient( Papp ) in four intestinal regions were calculated.Results Ka and Papp of euphorbia factor L1 at colon were the highest of the whole rat intestine.Significant increase of Ka and Papp showed at rat colon when co-perfused with verapamil hydrochloride, by contrast, decrease of Ka and Papp found when co-perfused with indomethacin.Conclusion We inferred that verapamil hydrochloride be the substrate of P-gp and that indomethacin be not the substrate of MRP2 .关键词
单向肠灌流/千金子甾醇/P-糖蛋白/多药耐药相关蛋白/大鼠Key words
single-pass intestinal perfusion/euphorbia factor L1/P-glycoprotein/multidrug resistance-as-sociated protein/rats分类
医药卫生引用本文复制引用
张秀婷,王英姿,李韶菁,段飞鹏,王晴,张春泥,李凤英,李文华,骆声秀..在体单向肠灌流模型研究千金子甾醇在大鼠肠吸收特性[J].北京中医药大学学报,2015,(9):624-627,4.基金项目
国家自然科学基金资助项目（No．81274082），北京中医药大学协同创新建设计划 （）
