草业学报Issue(9)：89-95,7.DOI:10.11686/cyxb2014411
中国西北地区披碱草属植物所带内生真菌的培养特征
Characteristic of asexual endophytes isolated from Elymus species in northwest China
摘要
Abstract
Forage grasses (Poaceae)often harbour endophytes belonging to the Neotyphodium and Epichlo(Clavicipitaceae,Ascomycota)genera.Neotyphodium endophytes,the asexual state of Epichlospecies,helps to protect cool-season grasses against biotic and abiotic stresses including drought,disease,herbivory and para-sitism and enhance aboveground and belowground vegetative and reproductive growth.There is little informa-tion on colonial diversity of asexual endophytes isolated from Elymus species in northwest Chinese.In the present study,different geographic populations of Elymus species were tested using the aniline blue screening method;15 endophyte strains were isolated from endophyte positive plants.The average growth rate (on pota-to dextrose agar)of the isolated strains was 0.41-0.85 mm/day and was correlated with altitude.Endophyte strains isolated from regions below an altitude of 3000 m grew faster than strains isolated from regions above 3000 m.The colonial morphology of endophyte strains isolated from regions above 3000 m was similar whereas the colonial morphology of endophyte strains isolated from lower altitudes differed.In addition,the phyloge-netic trees showed that endophytes collected above 3000 m were clustered whereas endophyte strains from low-er altitudes demonstrated a star-like topology.The results will provide a theoretical basis for the identification of endophyte from northwest Chinese Elymus species.关键词
内生真菌/披碱草/菌落/多样性/系统发育Key words
endophyte/Elymus/colony/diversity/phylogeny引用本文复制引用
宋辉,南志标,田沛..中国西北地区披碱草属植物所带内生真菌的培养特征[J].草业学报,2015,(9):89-95,7.基金项目
国家“973”项目（2014CB138702）和中央高校基本科研业务费专项资金（lzujbky-2014-76，lzujbky-2014-81）资助。 （）
