草业学报Issue(9)：96-103,8.DOI:10.11686/cyxb2015026
两株紫花苜蓿根际芽孢杆菌的筛选及生防效果研究
Isolation,identification,and biocontrol effects ofBacillus spp.from the rhizosphere of alfalfa
摘要
Abstract
The aims of this study were to identify two Bacillus spp.,CYY-6 and CYY-42,isolated from the rhizosphere of alfalfa,and to evaluate their ability to inhibit Fusarium oxysporum,the causal pathogen of alfal-fa root rot.Based on physiological,biochemical,morphological,and 16S rDNA sequencing analyses,strain CYY-6 was identified as Bacillus licheniformis and CYY-42 as Brevibacillus brevis.Both strains were able to synthesize siderophores,although at different levels.In a dual culture assay,both strains inhibited the growth of F.oxysporum.Based on the inhibition zones,the inhibitory rates of CYY-6 and CYY-42 against F.oxyspo-rum were 49.3% and 56.3%,respectively.The fermentation broth of each strain resulted in deformed growth of the mycelia and spores of F.oxysporum.The mycelia were twisted,broken,or fragmented,and the num-ber of spores was significantly decreased.Strains CYY-6 and CYY-42 showed 57.55% and 64.03% inhibition of alfalfa root rot in pot experiments,indicating that both strains have potential applications in controlling alfal-fa root rot.关键词
芽孢杆菌/紫花苜蓿/生物防治/尖孢镰刀菌/根腐病Key words
Bacillus spp./alfalfa (Medicago sativa)/biocontrol/Fusarium oxysporum/root rot引用本文复制引用
刘莎莎,程园园,张丹,王晓丹,刘佳莉,郭长虹..两株紫花苜蓿根际芽孢杆菌的筛选及生防效果研究[J].草业学报,2015,(9):96-103,8.基金项目
国家自然科学基金项目（No．31470571；31170479），国家科技支撑计划项目（No．2011BAD17B04-2-1），黑龙江省科技攻关项目（No． GC12B304）和黑龙江省自然科学基金项目（No．C201142）资助。 （）
