动物医学进展Issue(10)：43-47,5.
基因工程菌 K88-K99融合蛋白高密度发酵工艺的初步研究
Preliminary Study on High Density Fermentation Thchnology of Gene Engineering Bacteria K88-K99 Fusion Protein
摘要
Abstract
The five kinds of media,LB,improved LB,improved LB+ trace salt solution,improved Minca and 2×YT+10 g/L glucose were screened,and the initial pH of medium were set as 6.0,6.5,7.0 and 7.5 for protein expression.With automatic microorganism growth instrument,OD values of bacterial liquid were measured every half an hour.Finally 2 × YT + 5 g/L glucose and pH7.0 were confirmed for the growth of bacterial protein expression.The optimal induction time was in 3.0 h-3.5 h after culture by bac-terial growth curve,induction time 4.0 h,oxygen control at 30%,for ETEC E.coli to provide basic data for the research of high density fermentation.关键词
K88-99/培养基/高密度发酵/融合蛋白Key words
K88-99/culture medium/high-density fermentation/fusion protein分类
农业科技引用本文复制引用
谢金文,李书光,肖跃强,王文秀,唐娜,董林,李峰,沈志强..基因工程菌 K88-K99融合蛋白高密度发酵工艺的初步研究[J].动物医学进展,2015,(10):43-47,5.基金项目
山东省自然科学基金青年基金项目（ZR2012CQ012，ZR2013CQ006，ZR2014CQ009，ZR2014CQ010）；山东省现代农业产业技术体系生猪创新团队项目（SDAIT-06-011-14）；滨州市2013年科学技术发展计划项目 （）
