南方医科大学学报Issue(9)：1245-1250,6.
核因子I-C降低皮肤成纤维细胞对TGF-β的敏感性
Nuclear factor I-C inhibits platelet-derived growth factor-induced enhancement of dermal fibroblast sensitivity to TGF-β
摘要
Abstract
Objective To investigate the effect of nuclear factor I-C (NFI-C) on platelet-derived growth factor (PDGF)-induced up-regulation of TGF-βreceptor II (TβRII) in dermal fibroblasts. Methods A lentiviral vector containing NFI-C sequence (Lenti-GFP-NFI-C) was transfected into a human foreskin fibroblast cell line (HFF-1). Cultured HFF-1 cells, cells transfected with Lenti-GFP-NFI-C, and cells transfected with a negative virus were stimulated with PDGF-BB, and Western blotting and RT-qPCR were used to detect the expression levels of TβRII in the treated cells. Results PDGF treatment significantly increased the expression level of TβRII in HFF-1 cells (P<0.05). The cells transfected with Lenti-GFP-NFI-C expressed a significantly lower level of TβRII than non-transfected cells in response to PDGF stimulation (P<0.05), but the negative virus showed no such inhibitory effect (P>0.05). No significant difference was found in the expression level of TβRII protein between cells transfected with Lenti-GFP-NFI-C-transfection before PDGF stimulation and the blank control cells. Conclusion NFI-C can inhibit PDGF-induced up-regulation of TβRII and thus reduce the sensitivity of the dermal fibroblasts to TGF-β.关键词
核因子I-C/血小板源性生长因子/转化生长因子-β/转化生长因子-β受体Ⅱ/瘢痕Key words
nuclear factor I-C/platelet-derived growth factor/transforming growth factor-β/transforming growth factor-βreceptor II/scar引用本文复制引用
张亮平,王洋,雷睿,沈辉,沈毅忱,吴智南,徐靖宏..核因子I-C降低皮肤成纤维细胞对TGF-β的敏感性[J].南方医科大学学报,2015,(9):1245-1250,6.基金项目
国家自然科学基金（81471874，81171815）；浙江省科技计划项目（2012C23080） Supported by National Natural Science Foundation of China (81471874,81171815) （81471874,81171815）
