福建农业学报Issue(7):631-635,5.
大黄鱼内脏白点病的病原分析与鉴定
Identification of Pathogen Responsible for White-spots Disease in Larimichthys crocea
摘要
Abstract
Two strains of the suspected bacteria ,H2013032002 and H2013032003 ,were isolated from croakers , Larimichthys crocea ,in Sanduao ,Fujian .An artificial infection with the isolates caused the similar white‐spots disease in the fish .Subsequently ,morphological and biochemical tests identified the isolated strains as Pseudomonas putida or P . f luorescent . Then , the 16S‐rDNA sequence analysis showed that the bacteria had a 99.0%homologous rate with P .putida .In addition ,a drug sensitivity test revealed that the two strains of pathogen were highly sensitive to norfloxacin ,ciprofloxacin ,oxolinic acid and kanamycin neomycin .It was ,therefore ,determined that P .putid was likely the culprit that caused the white‐spots disease in croakers .关键词
大黄鱼/内脏白点病/恶臭假单胞菌/16S rRNA/药物敏感Key words
Larimichthys crocea/white-spots disease/Pseudomonas putida/isolation and identification分类
农业科技引用本文复制引用
许斌福,程海华,池洪树,方冬兰,柯巧珍,韩坤煌,王凡,龚晖..大黄鱼内脏白点病的病原分析与鉴定[J].福建农业学报,2015,(7):631-635,5.基金项目
福建省科技计划项目(2014N0010);福建省农业科学院科研项目(工程化实验室类)(2012DG-5);福建省财政专项---福建省农业科学院科技创新团队建设项目 ()