福建农业学报Issue(7)：693-696,4.
杆菌肽产生菌的 ART P诱变育种及培养基优化
Breeding of Bacillus licheni f ormis Mutant by ARTP and Optimization of Bacitracin-producing Process
摘要
Abstract
The bacitracin‐producing Bacillus licheniformis ZZ061 was isolated from an aquaculture facility .From the isolated bacteria , mutants with enhanced capability of generating bacitrcin were bred by means of ARTP mutagenesis .In the process ,Micrococcus luteus was used as the indicator bacterium ,and the bacteriostatic circle method employed for the screening .One of the mutants ,SF1406 ,showed the greatest increase ,i .e .,130% higher than the wild strain , in its bacitrcin productivity . Subsequently , an orthogonal experiment was conducted to optimize the culture medium formulation .It was found that the medium consisting of 3% corn starch ,1.5%ammonium sulfate ,1% soybean powder and 3% sucrose rendered the most desirable results with an additional 44%productivity increase on the yield of 810 U · mL -1 .关键词
地衣芽孢杆菌/杆菌肽/ARTP诱变/优化Key words
Bacillus licheniformis/bacitracin/ARTP mutagenesis/optimization分类
农业科技引用本文复制引用
罗秀针,林燕燕..杆菌肽产生菌的 ART P诱变育种及培养基优化[J].福建农业学报,2015,(7):693-696,4.基金项目
福建省教育厅B类项目 （）
