福建农业学报Issue(7)：709-717,9.
副溶血弧菌检测方法研究进展
Advance in Methodology Development for Detecting Vibrio parahaemolyticus
摘要
Abstract
Vibrio parahaemolyticus (Vp) is a halophilic gram‐negative bacterium .It widely exists in warm water fishes ,shrimps and shellfishes .The seafood carriers ,when ingested ,can cause the typical gastroenteritis symptoms including diarrhea ,intestinal cramps ,nausea and vomiting .When a patient is seriously infected ,septicemia and kidney failure may result .The rate of the food poisoning incidents caused by V p has increased drastically in recent years ,reaching the top of the list among all foodborne illnesses .With the wide‐spread nature and seriousness of the diseases in humans ,the need for a rapid ,sensitive ,low‐cost and effective detection method has become apparent . Methodologies based on the virulence factors that apply the molecular biology techniques including PCR ,Loop‐mediated isothermal amplification ,nucleic acid probe and gene chip ,as well as the immunology tools ,such as ELISA and the immune colloidal gold ,are being explored .This article evaluates the relevant methods to provide a comprehensive reference for the related studies .关键词
副溶血弧菌/检测方法/PCR/ELISAKey words
Vibrio parahaemolyticus (Vp)/detection method/PCR/ELISA分类
生物科学引用本文复制引用
温灿,顾小松,王荣智..副溶血弧菌检测方法研究进展[J].福建农业学报,2015,(7):709-717,9.基金项目
福建农林大学毕业生科研启动项目 A 类博士科研启动基金（132130001）；福建农林大学新青年教师科研基金 （）
