高等学校化学学报Issue(9)：1687-1693,7.DOI:10.7503/cjcu20150133
基于纳米金复合探针-蛋白芯片检测心肌损伤标志物
Detection of Myocardial Injury Markers Based on Double Gold Nanoparticles Probes and Protein Chip
摘要
Abstract
A novel method based on gold nanoparticles( AuNPs) probes and protein chip was developed to de-tect myocardial injury markers. Two AuNPs probes were constructed:one was modified with detection antibody and DNA probe 1(detection probe), and the other was labeled with DNA probe 2 which was complementary to DNA probe 1(signal probe). When target antigen was present, a sandwich structure(capture antibody-target antigen-detection probe-signal probe) was formed through the interaction of the antibody-antigen and the hy-bridization of complemenatry DNAs. Then the chip was dyed by the gold deposition solution, and the signal was amplified. The results were observed with a microscope linked with an image analysis software. This sys-tem could simultaneously detect multiple markers in 40 min. The detection limit of cTnⅠ was 10 pg/mL, which was equivalent to ECLIA in clinical. For HFABP and MYO, the detection limit could be as low as 640 and 10 pg/mL, respectively, and the sensitivity was improved greatly when compared with ECLIA and ELISA.关键词
纳米金探针/蛋白芯片/杂交/心肌损伤标志物Key words
Gold nanoparticles probe/Protein chip/Hybridization/Myocardial injury marker分类
化学化工引用本文复制引用
黄丽君,张丽华,毛红菊,王萍,蒋友旭,李盼盼,金庆辉,赵建龙..基于纳米金复合探针-蛋白芯片检测心肌损伤标志物[J].高等学校化学学报,2015,(9):1687-1693,7.基金项目
国家“九七三”计划项目(批准号:2012CB933303)和上海市科学技术委员会生物医药项目(批准号:12441902600)资助 （批准号:2012CB933303）
