高等学校化学学报Issue(9)：1707-1712,6.DOI:10.7503/cjcu20150115
白藜芦醇-大麦醇溶蛋白复合纳米颗粒的光稳定性、缓释行为及抗氧化能力
Light Stability, Controlled-release and Antioxidation of Resveratrol-hordein Composite Nanoparticles
摘要
Abstract
In order to protect the physiological activity of resveratrol, resveratrol-hordein nanoparticles were self-assembled by liquid-liquid dispersion method. The mean diameter of the nanoparticles [ ( 135. 0 ± 2. 5 ) nm] was measured with dynamic light scattering method, with encapsulation efficiency of resveratrol(90. 4%) and drug loading(18. 8%) were obtained by ultraviolet spectrophotometry method. Scanning electron micro-scope( SEM) showed that the morphology of nanoparticles was smooth and spherical. The effect of composite nanoparticles carrier type on the light stability of resvertrol was investigated. The results showed that the stabil-ity of resveratrol in nanoparticles was improved by 26% compared with that of free resveratrol after ultraviolet irradiation for 18 h. Resveratrol release behavior was studied in the simulated gastro-intestinal tract. The data revealed that 35% resveratrol was released from resveratrol-hordein nanoparticles in simulated gastric fluid at the first 2 h, and then released slowly, until 56% resveratrol was released after 6 h. While in the simulated intestinal fluid, 42% resveratrol was released in the first 2 h, and then steadily released from nanoparticles ac-cording to zero-order release kinetics during the first 4 h. After 6 h, 88% resveratrol was released. The an-tioxidant capability of free resvertrol and composite nanoparticles was studied. 1,1-Diphenyl-2-picrylhydrazyl radical( DPPH) experiment results showed that resvertrol in composite nanoparticles had a little more scaven-ging effect on DPPH free radicals( IC50=0. 4182 mmol/L) compared with free resvertrol( IC50=0. 4378 mmol/L) . A HepG2 cellular antioxidant activity( CAA) assay further demonstrated the above results.关键词
白藜芦醇/大麦醇溶蛋白/纳米颗粒/自组装Key words
Resveratrol/Hordein/Nanoparticle/Self-assembly分类
化学化工引用本文复制引用
管骁,殷婷,韩飞..白藜芦醇-大麦醇溶蛋白复合纳米颗粒的光稳定性、缓释行为及抗氧化能力[J].高等学校化学学报,2015,(9):1707-1712,6.基金项目
国家自然科学基金(批准号:31101348)和上海市自然科学基金(批准号:14ZR1419200)资助 （批准号:31101348）
