广东牙病防治Issue(10)：509-513,5.
三维培养成骨细胞压应力作用下的破骨诱导机制研究∗
Study of the osteoclast induction mechanism of three-dimensional culture osteoblasts under compressive stress
摘要
Abstract
Objective To investigate the expression change of key genes in osteoblasts under stress and induction of it on the osteoclast precursors in order to reveal the adjustment mechanism of the compressive stress on the bone resorption. Methods The mouse osteoblastic cell line MC3T3-E1 was put into typeⅠcollagen gel three-dimensional culture system and given 2 g/cm2 compressive stress load. Cell morphology was observed by fluorescence confocal microscopy. The ex-pression changes of receptor activator for nuclear factor-κB ligand ( RANKL) and osteoprotegerin ( OPG) after stress load was analyzed by quantitative real-time PCR and ELISA. The mouse monocyte-macrophage cells RAW264. 7 was induced cultured by MC3T3-E1 culture supernatants under stress, whose tartrate-resistant acid phosphatase, TRAP activity change was detected after the induced culture. Results Under compressive stress load, MC3T3-E1 kept an obvious three-di-mensional growth morphology, RANKL mRNA expression was significantly up-regulated ( P<0. 05) , whereas OPG mR-NA expression was inhibited ( P<0. 05); MC3T3-E1 RANKL concentration in the culture supernatant increased ( P<0. 05) , there was no significant change on the OPG concentration( P >0. 05); MC3T3-E1 culture supernatants under compressive stress load could promote the improvement of RAW264. 7 TRAP activity ( P<0. 05) . Conclusion Stress can promote osteoclasts differentiation by inducing osteoblasts up-regulating RANKL expression and inhibiting OPG ex-pression.关键词
压应力/成骨细胞/破骨细胞Key words
Compressive stress/Osteoblast/Osteoclast分类
医药卫生引用本文复制引用
耿远明,申晓青,徐平平..三维培养成骨细胞压应力作用下的破骨诱导机制研究∗[J].广东牙病防治,2015,(10):509-513,5.基金项目
国家自然科学基金(81271187) （81271187）
广东省科技计划项目(2011B080701099,2013B021800319) （2011B080701099,2013B021800319）
