广西植物Issue(5)：709-714,774,7.DOI:10.11931/guihaia.gxzw201406024
多年生黑麦草再生体系的建立
Establishment of tissue regeneration system of Lolium perenne
摘要
Abstract
The caryopsis of perennial ryegrass(Lolium perenne)cultivar‘Top Hat 2’was used as explants.Firstly, this paper focused on the effect of four ways of explants treatments (embryo end half a caryopsis,slitting embryo end half a caryopsis,small pieces of embryo end for caryopsis,small pieces of caryopsis)and two ways of surface sterili-zation methods(disinfection with sodium hypochlorite solution,disinfection with mercuric chloride)on callus induc-tion;Secondly,we studied the effects of different plant growth regulators(2,4-D and 6-BA)and their concentrations on callus induction by randomized block design of two factors;Thirdly,we studied the effects of different plant growth regulators(NAA,6-BA and ZT)and their concentrations on adventitious bud differentiation by orthogonal test of three factors and four levels;At last,we separately studied the effects of 0.10 mg•L-1 NAA and IBA on ad-ventitious root induction.The results were as follows:Under the same conditions,explants of full end half a caryop-sis embryos were surface sterilized with 75% alcohol for 1 min,then treated with sodium hypochlorite solution 1 mL•L-1 Tween 20 (the chlorine content>10%)for 30 min,the rate of callus induction were better than the other’s;The explants inoculated on MS medium with 5 mg•L-1 2,4-D and 0.03 mg•L-1 6-BA (pH=5.8),provid-ed us a relatively high induction rate of 68.7% and high quality callus;The most high differentiation rate was 56%when adventitious buds were inoculated in MS with 0.5 mg•L-1 NAA,0.5 mg•L-1 6-BA and 0.9 mg•L-1 ZT.The best rooting medium was 1/2MS+IBA with nothing plant growth regulator obtaining stout root,then the regenera-tion rate was 90%.This paper has established efficient tissue culture regeneration system for perennial ryegrass and laid a good foundation for conducting highly efficient genetic engineering.关键词
多年生黑麦草/组织培养/外植体/诱导/再生Key words
Lolium perenne/tissue culture/explants/induction/regeneration分类
农业科技引用本文复制引用
魏树强,钱永强,刘俊祥,孙振元..多年生黑麦草再生体系的建立[J].广西植物,2015,(5):709-714,774,7.基金项目
国家“863”项目(2011AA10020902) （2011AA10020902）
