广西植物Issue(5)：715-720,6.DOI:10.11931/guihaia.gxzw201409036
广藿香叶盘法高效再生体系主要影响因素的研究
Main influential factors for efficient plant regeneration system established from leaf-disc of Pogostemon cablin
摘要
Abstract
Pogostemon cablin syn.P.patchouli,is a perennial bushy herb introduced from Southeast Asia and has been extensively cultivated in South China,for both utilization in pharmaceutical and perfume industry.Patchouli cul-tivated in different regions has diverse morphological characteristics and essential oil constituents,which infect its therapeutic properties directly.It is necessary to established a cultivar with high quality of essential oil.This study aims to establish an efficient system of plant regeneration from the leaf-disc of Pogostemon cablin.The leaves of in vitro patchouli seedlings were used as materials.The effects of donor plant’s age,leaf position and the concentration of plant growth substances used single or combined on plant regeneration of P.cablin were studied.The results showed that low concentration’s benzylaminopurine (BA)combined with naphthaleneacetic acid(NAA)induced the high frequency of prolific adventitious shoots regeneration directly from leaf with less or without callus,the age of do-nor plant and position of leaf on stem both effected the frequency of shoot regeneration and the mean number of shoots.The high efficient plant regeneration system was established from leaf disc explants prepared from the leaves of the second nodes of thirty-day old-in vitro plant.The culture medium for the prolific shoot inducing was MS cul-ture medium supplemented with 0.1 mg•L-1 NAA and 0.5 mg•L-1 BA.The regeneration frequency was 100% and the maximum mean number of shoot per leaf disc was 96.5.Regenerated shoots were elongated and rooted on the half strength MS medium with 0.5 mg•L-1 GA3 and 0.1 mg•L-1 NAA.The rooting frequency was 100%.The plantlets with well-developed roots were successfully acclimatized in the greenhouse,and planted in the field with a survival rate of 96%.The established plant regeration system from leaf-disc of P.cablin would lay foundation for the gene transformation of P.cablin and its fast breeding for good cultivars.关键词
广藿香/叶盘/植株再生Key words
Pogostemon cablin/leaf-disc/plant regeneration分类
农业科技引用本文复制引用
莫小路,欧阳蒲月,曾庆钱,黄珊珊..广藿香叶盘法高效再生体系主要影响因素的研究[J].广西植物,2015,(5):715-720,6.基金项目
广东省科技厅粤港关键领域重点突破项目(2009A030901011) （2009A030901011）
广州市科技计划基础研究项目(2009J1-C201) （2009J1-C201）
广东食品药品职业学院自然科学研究项目(2012YZ002) （2012YZ002）
