广西植物Issue(5)：733-740,8.DOI:10.11931/guihaia.gxzw201410003
黄独微型块茎低温保存及其萌发苗遗传稳定性研究
Low temperature conservation of Dioscorea bulbifera microtuber and genetic stability of its germination seedling
摘要
Abstract
Using Dioscorea bulbifera microtubers as material,the anatomical structure and the physiological and bio-chemical indexes of microtubers during low-temperature conservation were observed and measured,the genetic stabil-ity of germination seedlings after low-temperature conservation was also tested in this article.The results were as fol-lows:HE staining method was complicated,whose effect was difficult to observe.Compared with HE staining meth-od,safranin fast green staining was much simpler,whose effect was better.Therefore,Safranin-fast green method was more suitable for staining of D.bulbifera microtubers;Without low temperature conservation,the starch grains in the cells around D.bulbifera microtuber has not begun to be exploited and be still conserved.When D.bulbifera microtuber treated with low temperature,the starch grains in the cells surrounding microtuber began to disappear.The longer the conservation time is,the more the cell number with no starch grains also increased.The starch grains in cells had started to translate into other substances for cell life activities during low temperature stor-age.When conserving for 90 d,the cell whose starch grains disappeared extended to the middle of the microtuber;During the low temperature conservation period,the antioxidase,the amylase activity,the proline content and the sol-uble sugar content of D.bulbifera microtubers all showed an increasing trend;During the low temperature conserva-tion of D.bulbifera microtubers for 0-90 d,SOD activity during 18-54 d continued to increase,and SOD activity during 54-90 d still remained unchanged;POD activity increased significantly within 18-36 d,maintained stability within 3-54 d,increased significantly during 54-72 d,kept stable again in 72-90 d;CAT activity trends is consist-ent with the SOD activity,which continued to increase in 18-54 d,and remain unchanged in 54-90 d;α-amylase and total amylase activity increased rapidly in 18-36 d,had no significant change in 36-54 d and continuously increased significantl in 54-90 d;β-amylase activity increased significantly in 18-54 d,maintained stability within the 54-72 d,and began to increase significantly within the 72- 90 d;Soluble sugar content increased significantly within 18-36 d,had no significant change within 36-54 d,and began to increase significantly during 54-90 d;Proline content had no change in 18-36 d,increased significantly in 36-72 d,and remained unchanged in 72-90 d.There was no signif-icant difference between the seedling germinated from D.bulbifera microtubers stored at low temperature for 90 d and the seedling germinated from D.bulbifera microtubers without low temperature conservation in morphology (average plantlet length,average leaf number,average interstem length and average root number),DNA content, physiological index (total chlorophyll content,superoxidase dismutase activity,catalase activity,peroxidase activity, soluble sugar content and soluble protein content)and its leaf photosynthetic characteristics parameters (net photo-synthetic rate,stomatal conductance,intercellular CO2 concentration,transpiration rate,stomatal limitation value, water use efficiency and instantaneous carboxylation rate)and chlorophyll fluorescence parameters (initial fluores-cence,maximal fluorescence,the most photochemical efficiency of PS II,the potential photochemical efficiency of PS II,the actual photochemical efficiency of PS II,captured excitation energy efficiency of open PSII reaction center,pho-tochemical fluorescence quenching coefficient and non-photochemical fluorescence quenching coefficient),which indi-cated that low temperature conservation of D.bulbifera microtuber could not cause genetic variation of the seedling.关键词
低温保存/黄独/微型块茎/解剖结构/生理生化指标/遗传稳定性Key words
low temperature conservation/Dioscorea bulbifera L./microtuber/anatomical structure/physiological and biochemical index/genetic stability分类
农业科技引用本文复制引用
尹明华,洪森荣,夏瑾华,林国卫,王爱斌,柯维忠..黄独微型块茎低温保存及其萌发苗遗传稳定性研究[J].广西植物,2015,(5):733-740,8.基金项目
国家自然科学基金(31360072) （31360072）
江西省教育厅科学技术研究项目(GJJ14713) （GJJ14713）
