哈尔滨商业大学学报（自然科学版）Issue(4)：393-397,5.
流式细胞仪检测线粒体膜电位方法的研究
Study on methods for detecting mitochondrial membrane potential by flow cytometry
岳磊 1张垚 1张楠曦1
作者信息
- 1. 哈尔滨工业大学生命科学与技术学院，哈尔滨150080
- 折叠
摘要
Abstract
This paper aimed to determine the applicable conditions for detecting mitochondri -al membrane potential (Δψm ) of fluorescent dyes by flow cytometry .Rhodamine 123, Di-OC6 (3) , TMRE, JC-1 were chose and used to analyze the conditions of each dye for the mitochondria membrane potential detection through the induction of UV .The results showed that the change of Δψm detected by Rhodamine 123 was larger than other dyes , which may be related to the adaption of Rhodamine 123 to acute cell damage .By comparing the experi-mental method , it is more accurate for the future of the mitochondrial membrane potential de-tection by flow cytometry .关键词
流式细胞仪/线粒体膜电位/罗丹明123/DiOC6(3)/TMRE/JC-1Key words
flow cytometry/mitochondrial membrane potential/Rhodamine 123/DiOC6(3)/TMRE/JC-1分类
医药卫生引用本文复制引用
岳磊,张垚,张楠曦..流式细胞仪检测线粒体膜电位方法的研究[J].哈尔滨商业大学学报（自然科学版）,2015,(4):393-397,5.
