海洋科学Issue(8)：1-6,6.DOI:10.11759/hykx20140423003
浒苔原生质体再生苗对草丁膦与6种抗生素的敏感性
Sensitivity of protoplast-regenerated seedling ofUlva prolifera to phosphinothricin and six kinds of antibiotics
摘要
Abstract
Ulva proliferais the dominant species of green tides in the Yellow Sea, which can accumulate a large amount of biomass in a short period. Establishing the genetic transformation system ofU. proliferacan provide a powerful tool for verifying the function of related genes. In this paper, the sensitivity of protoplast-regenerated seedling ofU. prolifera to phosphinothricin and six kinds of antibiotics was tested. Results showed that seedlings were not sensitive to ampicillin, kanamycin, streptomycin sulphate and zeocin, weakly sensitive to chloramphenicol and hygromycin, but very sensitive to phosphinothricin. Moreover, the sensitivity to phosphinothricin was signifi-cantly correlated with photoperiod. The LD50 and 95% confidence range of phosphinothricin were calculated. These results can lay basis for screening transformants and establishing the genetic transformation system ofU. prolifera.关键词
浒苔(Ulva prolifera)/基因工程/草丁膦/抗生素/敏感性/光周期Key words
Ulva prolifera/genetic engineering/phosphinothricin/antibiotics/sensitivity/photoperiod分类
农业科技引用本文复制引用
吴春辉,王峰,赵瑾,刘晓杰,姜鹏..浒苔原生质体再生苗对草丁膦与6种抗生素的敏感性[J].海洋科学,2015,(8):1-6,6.基金项目
中国科学院海洋战略性先导科技专项(XDA11020304) （XDA11020304）
国家863计划资助项目(2014AA093501) （2014AA093501）
国家自然科学基金项目(31300-365) （31300-365）
海洋公益项目(201205010) （201205010）
山东省博士基金项目(2010BSB02009) （2010BSB02009）
