检验医学与临床Issue(19)：2817-2819,2822,4.DOI:10.3969/j.issn.1672-9455.2015.19.003
赖氨大黄酸通过调控miRNA表达水平抑制HBV增殖
RHL inhibiting proliferation of HBV in HepG2 .2 .15 cells through regulating the miRNA expression level
摘要
Abstract
Objective To study the effect and molecular mechanisms of rhein lysinate(RHL) on the prolifera‐tion of hepatitis B virus(HBV) and viral antigen expression to establish the preliminary experimental evidence for re‐searching the anti‐HBV mechanism of RHL .Methods HepG2 .2 .15 cells were divided into the experimental groups (adding 5 ,10 ,20 ,40 ,80 μmol/L RHL) and the control group without adding RHL .After culturing for 24 ,48 h ,the supernatant was obtained .The cell proliferation activity was detected by the non‐radioactive cell proliferation assay (M TS) ,the expression level of HBsAg and HBeAg were detected by adopting the ELISA method ,the HBV DNA copy number and the expression levels of miR‐210 ,miR‐185 ,miR‐199a‐3p ,miR‐370 ,miR‐196a ,miR‐184 and miR‐217 were detected by real time quantitative PCR(RT PCT ) .HepG2 .2 .15 was transfected by miR‐210 ,miR‐185 ,miR‐199a‐3p ,miR‐370 ,miR‐196a ,miR‐184 and miR‐217ASO ,the cell proliferation activity was detected by the M TS method ,the expression levels of HBsAg and HBeAg were detected by rthe enzyme linked immunosorbent assay (ELISA) ,respectively .Results Without generating the effect on the cell proliferation activity ,RHL inhibited the generation of HBsAg and the HBV proliferation .The expression level of miR‐210 ,miR‐185 and miR‐199a‐3p was in‐creased by RHL(20 μmol/L) and the expression level of miR‐370 was inhibited by RHL(20 μmol/L) ,the differences compared with the control group were statistically significant(P < 0 .05) .Conclusion RHL is possible to suppress HBV proliferation and HBsAg generation by affecting the miRNA expression in HepG2 .2 .15 cells .关键词
赖氨大黄酸/乙型肝炎病毒/乙型肝炎病毒表面抗原/miRNAKey words
rhein lysinate/hepatitis B virus/hepatitis B surface antigen/miRNA引用本文复制引用
张荣花,王密斯,王琳,冯雪研,郝晓方,甄永占,李琳,陈静,章广玲..赖氨大黄酸通过调控miRNA表达水平抑制HBV增殖[J].检验医学与临床,2015,(19):2817-2819,2822,4.基金项目
国家自然科学青年基金资助项目（81201281）；河北省自然科学基金资助项目（H2013209180、C2012401037、H2013209040）；河北省唐山市科技计划资助项目（12140209A-4）；华北理工大学研究生创新项目（2015S16、2015S17）；国家级、省级大学生创新项目（201410081021）。 （）
