临床与实验病理学杂志Issue(8)：880-884,5.DOI:10.13315/j.cnki.cjcep.2015.08.010
肺癌组织及其肺癌干细胞中FOXO3的表达及意义
FOXO3 expression in lung tissue and lung cancer stem cells and its implication
张志强 1杨艳荣 1李运霞1
作者信息
- 1. 新乡医学院第一附属医院呼吸科，新乡 453100
- 折叠
摘要
Abstract
Purpose To detect the expression of FOXO3 in lung cancer specimens and lung cancer stem cell, for diagnosis and treat-ment of lung cancer. Methods Immunohistochemistry, semi-quantitative PCR and Western blot were used to detect FOXO3 protein expression in clinical specimens of lung cancer. PCR and Western blot verified FOXO3 mRNA and protein expression of lung cancer cells. Results Immunohistochemistry showed that the FOXO3 was significantly lower expressed in lung cancer tissues and the corre-sponding lung cancer stem cells;Detection of FOXO3 mRNA and protein by RT-PCR and Western blot showed both had significantly lower expression;as progression of the disease, there was a clear decreasing trend, in which negative expression rate of FOXO3 protein accounted for 66. 7% in all patients;at the same times, the rate of the expression gradually decreased with loss of differentiation and metastasis of the tumor. Conclusions FOXO3 shows low expression in lung tissue and lung cancer stem cells, while it is closely relat-ed to the degree of malignancy and lung cancer metastasis, which may be a new target for the treatment.关键词
肺肿瘤/FOXO3/肺癌干细胞/RT-PCR/Western blotKey words
lung neoplasms/FOXO3/cancer stem cells/RT-PCR/Western blot分类
医药卫生引用本文复制引用
张志强,杨艳荣,李运霞..肺癌组织及其肺癌干细胞中FOXO3的表达及意义[J].临床与实验病理学杂志,2015,(8):880-884,5.
