临床与实验病理学杂志Issue(8)：900-903,4.DOI:10.13315/j.cnki.cjcep.2015.08.014
SOCS3在糖尿病小鼠肾小管间质病变中的作用
Role of SOCS3 in tubulointerstitial injury of mice with diabetes
摘要
Abstract
Purpose To investigate the role of SOCS3 on diabetic renal injury. Methods Male CD-1 mice were randomly divided into four groups:control group, diabetic group, empty plasmid vector transfection group and SOCS3 plasmid transfection group. The diabet-ic mice were induced by intraperitoneal injection of STZ at a dose of 150 mg/kg body weight. The mice of transfection group were re-ceived an injection of SOCS3 plasmid or empty vector at every 7 days thereafter. Specimens were collected at 12 week after STZ injec-tion. The morphological changes of tubular epithelial cells were observed by transmission electron microscope. RT-PCR and immuno-histochemistry were used to determine the mRNA and protein expression of CK18 and α-SMA. Western blotting analysis was used to determine the protein expression of SOCS3, p-STAT3, CK18 and α-SMA. Results SOCS3 overexpression in kidney down-regulated the levels of p-STAT3 andα-SMA but up-regulated the expression of CK18. Conclusion Overexpression of SOCS3 can ameliorate the tubular epithelial-mesenchymal transdifferentiation of diabetic mice via inhibiting the phosphorylation of STAT3.关键词
糖尿病肾病/肾小管上皮细胞/细胞因子信号传导抑制蛋白3/信号转导和转录活化因子3Key words
diabetic nephropathy/tubular epithelial cell/SOCS3/STAT3分类
医药卫生引用本文复制引用
高翔,邢玲玲,刘淑霞,杜云霞,李科军,刘思媛,刘青娟..SOCS3在糖尿病小鼠肾小管间质病变中的作用[J].临床与实验病理学杂志,2015,(8):900-903,4.基金项目
教育部高等学校博士学科点专项科研基金(新教师类2013132320001) （新教师类2013132320001）
