临床与实验病理学杂志Issue(8)：904-907,4.DOI:10.13315/j.cnki.cjcep.2015.08.015
细胞块联合免疫组化在胸腔积液诊断中的应用
Application of cell block combined with immunohistochemistry in diagnosis of pleural effusion
摘要
Abstract
Purpose To investigate the application value in diagnosis of pleural effusion by cell block combined with immunohisto-chemistry. Methods 60 cases of pleural effusion were collected, and paraffin-embedded cell block was prepared and immunohisto-chemistry was used to detect the expression of CK7, TTF-1, E-cadherin, CEA and Calretinin. Results By use of cell block combined with immunohistochemistry, malignant detected rate was higher than that of the conventional centrifugal smear. There was statistical significance in the expression of CK7, TTF-1, E-cadherin, CEA and Calretinin between pleural effusion lung adenocarcinoma and reac-tive hyperplasia mesothelial cells (P<0. 05). CK7, TTF-1, E-cadherin and CEA were highly expressed in pleural effusion of lung ad-enocarcinoma cell. Calretinin was highly expressed in hyperplastic mesothelial cells. Conclusion Cell block and immunohistochemi-cal technique combination in the differential diagnosis of difficult pleural effusion has important clinical significance. It is worthy of popularization and further clinical application.关键词
肺腺癌细胞/胸腔积液/免疫组织化学/细胞块Key words
lung adenocarcinoma cell/pleural effusion/immunohistochemistry/cell block分类
医药卫生引用本文复制引用
罗丽花,张婉仪,刘惠娟,李炳权,伍世钢,肖靖华..细胞块联合免疫组化在胸腔积液诊断中的应用[J].临床与实验病理学杂志,2015,(8):904-907,4.基金项目
广东省清远市科技计划项目(2013B034) （2013B034）
