临床与实验病理学杂志Issue(9)：966-970,5.DOI:10.13315/j.cnki.cjcep.2015.09.002
无血清培养有效富集乳腺癌干细胞
Enrichment of breast cancer stem cells by serum-free culture
摘要
Abstract
Purpose To identify whether serum-free culture can enrich breast cancer stem cells from MCF-7 human breast cancer cell line. Methods MCF-7 human breast cancer cell line by serum-free culture and serum culture technology were cultured, its cell mor-phology and growth pattern were observed by the inverted microscope. The expression of stem cell surface molecular makers CD24, CD44 was observed by the inverted fluorescence microscope and the flow cytometry, the proportion of different subpopulation cells was detected by the flow cytometry. At the same time, difference of the cell cycle was detected by the flow cytometry. Results The cell line cultured by serum-free culture grew in the form of suspended microspheres of different sizes in the medium, but the cell line cul-tured by serum culture grew in the form of monolayer adherent growth. There was no obvious difference in the expression of stem cell surface molecular makers CD44 between the suspended microsphere cells and the adherent cells, but the expression of CD24 in the sus-pended microsphere cells decreased compared to the adherent cells. The proportion of CD44 + /CD24 -/low phenotype cells in the suspen-ded microsphere cells and the adherent cells was (86. 93 ± 0. 53)% and (19. 98 ± 0. 62)%, respectively (P<0. 05), the proportion of CD44 + /CD24 + phenotype cells was (12. 68 ± 0. 59)% and (79. 90 ± 0. 57)%, respectively (P<0. 05). The proportion of mitotic cells in the suspended microsphere cells and the adherent cells was ( 18. 85 ± 2. 26 )% and ( 43. 91 ± 1. 81 )%, respectively ( P<0. 05), the proportion of quiescent cells was (64. 92 ± 2. 07)% and (39. 82 ± 1. 77)%, respectively (P<0. 05). Conclusion CD44 + /CD24 -/low breast cancer stem cells can be effectively enriched by the serum-free culture technology.关键词
乳腺肿瘤/干细胞/悬浮微球体细胞/CD44+/CD24-/low表型干细胞Key words
breast neoplasm/stem cell/suspended microsphere cells/CD44 + /CD24 -/low phenotype cells分类
医药卫生引用本文复制引用
尹燕雪,李传伟,于爱莲,王学春..无血清培养有效富集乳腺癌干细胞[J].临床与实验病理学杂志,2015,(9):966-970,5.基金项目
山东省科技攻关项目资助课题(2009GG20002047)、山东省医药卫生科技发展计划(2011HD015) （2009GG20002047）
