南华大学学报（自然科学版）Issue(3)：14-20,38,8.
浸矿微生物共培养体系在氟胁迫下的基因调控机理探索
Gene Regulation Mechanism of a Bioleaching Microbial Co-culture upon Fluoride Stress
摘要
Abstract
In order to explore the fluoride tolerance and the gene regulation mechanisms of mixed bioleaching microorganisms,the gene expression profile of a co-culture including five typical bioleaching strains ( Acidithiobacillus ferrooxidans ATCC 23270 , Leptospirillum fer-riphilum YSK,Sulfobacillus thermosulfidooxidans ST,Acidithiobacills thiooxidans A01,Acid-ithiobacills caldus S1 ) upon 4 . 8 mmol/L fluoride stress was investigated using the func-tional gene array. The results showed, the genes associated with fluoride stress were in-volved with sulfur metabolism,cell membrane,electron transport,detoxification,carbon fix-ation,nitrogen metabolism,and so on. Additionally,majority of genes was induced upon the short time ( <60 min) fluoride stress,while repressed upon the long time ( >120 min) fluoride stress. The effects of fluoride stress on different microbial population in the co-cul-ture were different. The results revealed that the dominant species in the co-culture played the crucial role for resisting fluoride stress,while the minor species to a large extent assis-ted the oxidation ability preservation and growth of the dominant species.关键词
浸矿微生物/共培养体系/氟胁迫/基因调控Key words
bioleaching microorganisms/co-culture/fluoride stress/gene regulation分类
矿业与冶金引用本文复制引用
李乾,孙静,丁德馨,王清良,马丽媛,刘学端..浸矿微生物共培养体系在氟胁迫下的基因调控机理探索[J].南华大学学报（自然科学版）,2015,(3):14-20,38,8.基金项目
湖南省自然科学基金资助项目(14JJ6024) （14JJ6024）
湖南省教育厅科研基金资助项目(13C821) （13C821）
南华大学博士科研启动基金资助项目(2012XQD05) （2012XQD05）
