山东农业科学Issue(9)：1-6,6.DOI:10.14083/j.issn.1001-4942.2015.09.001
禾谷镰刀菌致病性蛋白CYP51CcDNA克隆及生物信息学分析
Cloning and Bioinformatics Analysis of Pathogenic Protein CYP51C cDNA in Fusarium graminearum
摘要
Abstract
In this study,the full -length cDNA sequence of pathogenic protein CYP51C from Fusarium graminearum was cloned using RT -PCR,and the bioinformatics analysis of amino acid sequences was con-ducted and its typical characteristics was identified.The results showed that the full -length cDNA sequence of CYP51C included a complete open reading frame (ORF)of 1 554 bp,which encoded 517 amino acids.Its molecular weight was 58.6 kD and its isoelectric point was 6.36.It possessed the conserved domains which were characteristics of cytochrome P450 family members.It was not a secretory protein without a signal pep-tide,but was a hydrophilicity protein with transmembrane domain.The major structural elements of polypep-tide chain were α-helix and random coil.Subcellular localization result indicated that CYP51C was mainly located in cytoplasm.These results would provide references for the study on protein purification and protein structural biology,and then lay a foundation for drug design targeting pathogenic fungi.关键词
禾谷镰刀菌/14α脱甲基酶(CYP51)/基因克隆/致病性/生物信息学分析Key words
Fusarium graminearum/Sterol 14α-demethylation(CYP51)/Gene clone/Pathogenicity/Bioinformatics analysis分类
生物科学引用本文复制引用
孙晓梅,黄金光..禾谷镰刀菌致病性蛋白CYP51CcDNA克隆及生物信息学分析[J].山东农业科学,2015,(9):1-6,6.基金项目
国家自然科学基金项目“禾谷镰刀菌 CYP51蛋白抗药性分子机制”（31471735）；国家科技基础条件平台子项目“林业经济微生物资源标准化整理、整合”（2005DKA21207－17）；山东省“泰山学者”建设工程专项经费；青岛农业大学高层次人才科研基金项目 （）
